Trent University Graduate Thesis Collection

Item Description
Identifier
tula:etd
Type
Collection
Language
eng
Extent
1 item
Rights
Copyright for all items in the Trent University Graduate Thesis Collection is held by the author, with all rights reserved, unless otherwise noted.
Displaying 1 - 2 of 2

Results per page

Displaying 1 - 2 of 2

Mutation of the B10 Tyrosine and E11 Leucine in Giardia intestinalis Flavohemoglobin

22143-Thumbnail Image.png
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Lukaszewicz, Brian, Thesis advisor (ths): Rafferty, Steven P, Degree committee member (dgc): Saville, Barry J, Degree committee member (dgc): Yee, Janet, Degree granting institution (dgg): Trent University
Abstract: <p>The flavohemoglobin in Giardia intestinalis (gFlHb) is the only known protozoan member of a protein class typically associated with detoxifying nitric oxide (by oxidation to nitrate) in bacteria and yeast. Mutants of the B10 tyrosine (Y30F) and E11 leucine (L58A), conserved residues thought to influence ligand binding, were expressed and studied using Resonance Raman (RR) spectroscopy.… more

Characterisation of the Giardia Tata-Binding Protein - Preparation for an in vivo approach

22177-Thumbnail Image.png
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Kemp, Adam, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Saville, Barry J, Degree committee member (dgc): Brunetti, Craig R, Degree granting institution (dgg): Trent University
Abstract: <p>The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a… more