Trent University Graduate Thesis Collection

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tula:etd
Type
Collection
Language
eng
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1 item
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Copyright for all items in the Trent University Graduate Thesis Collection is held by the author, with all rights reserved, unless otherwise noted.
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Characterisation of the Giardia Tata-Binding Protein - Preparation for an in vivo approach

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Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Kemp, Adam, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Saville, Barry J, Degree committee member (dgc): Brunetti, Craig R, Degree granting institution (dgg): Trent University
Abstract: <p>The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a… more