English

The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a suitable yield and purity and used as the immunogen. The antibody was then tested for reactivity to the native protein in our laboratory. By Western blot analysis, it was possible to observe the enrichment of the gTBP within the nuclear fraction compared to a cytoplasmic fraction extracted from Giardia cells. However, the antibody could not be successfully used in an immunoprecipitation assay - suggesting that the antibody is unable to bind to the native structure of gTBP. Therefore, the focus of this work was changed to analyse gTBP via multiple sequence alignments, homology modelling and BLAST to identify any unique regions that may contribute to its unusual binding characteristics. These techniques were also used to identify specific regions of gTBP that may be used to generate synthetic peptides as immunogens for future antibody production.

Author Keywords: ChIP, Giardia intestinalis, Homology modelling, Immunoprecipitation, TATA-binding protein, Western Blotting

New conditions of materiality are emerging from fundamental changes in our ontological order. Digital subjectivity represents an emergent mode of subjectivity that is the effect of a more profound ontological drift that has taken place, and this bears significant repercussions for the practice and understanding of the political. This thesis pivots around mathematician Grigori 'Grisha' Perelman, most famous for his refusal to accept numerous prestigious prizes resulting from his proof of the Poincaré conjecture. The thesis shows the Perelman affair to be a fascinating instance of the rise of digital subjectivity as it strives to actualize a new hegemonic order. By tracing first the production of aesthetic works that represent Grigori Perelman in legacy media, the thesis demonstrates that there is a cultural imperative to represent Perelman as an abject figure. Additionally, his peculiar abjection is seen to arise from a challenge to the order of materiality defended by those with a vested interest in maintaining the stability of a hegemony identified with the normative regulatory power of the heteronormative matrix sustaining social relations in late capitalism. The first chapter analyses the formal aesthetics of legacy media representations of Perelman. The second chapter focuses on new media aesthetic productions pertaining to Perelman and the political ontology of digital media. The third chapter interrogates the political ontology of the materials utilized in Perelman's work and seeks to clarify the status of the conditions of the challenge of a digital hegemony.

Author Keywords: abjection, archive, autistic reason, digital subjectivity, Grigori Perelman, ontological drift

This thesis studies a subset of players of video games called "power-gamers" who play games in a way that mirrors labour as opposed to leisure. Through ethnographic fieldwork and exploration this thesis examines what constitutes "power-gaming" and seeks to unpack the differences between skill, fun, and labour. Chapter One analyzes how ethnographic fieldwork is performed in virtual worlds, and the necessary frameworks inherent to this. Chapter Two explores facets of technical hobbies, masculinity, skill, and how they relate to power-gaming. Chapter Three explores how different cultures globally choose to play-games, and the forms of sociability involved in this play. Chapter Four examines reality in relation to virtual worlds, and how players in virtual worlds explore and unpack their surroundings, which mirrors many scientific practices in the real world. Chapter Five explores narrative structure in games, and their relation to power-gaming practices. Chapter Six concludes with a discussion of power-gamers as a neo-liberal workforce.

Author Keywords: game design, neo-liberalism, playbour, power-gamer, sociability, virtual-ethnography

Phosphorus (P) is an essential macronutrient. In south-central Ontario, foliar P concentrations are low and studies have suggested that P may be limiting forest productivity. Current catchment mass balance estimates however, indicate that P is being retained suggesting that P should not be limiting to tree growth. Phosphorus deposition is measured using bulk deposition collectors, which are continuously open and therefore are subject to contamination by pollen and other biotic material with high P and potassium (K) concentrations and may therefore overestimate net P inputs to forested catchments. Average annual TP and K deposition at three long-term (1984 – 2013) monitoring sites near Dorset, Ontario ranged from 15 to 20 mg·m-2y-1 and 63 to 85 mg·m-2y-1, respectively, and was higher at Paint Lake compared with Plastic Lake and Heney Lake. Phosphorus and K in bulk precipitation were strongly positively correlated, but deposition patterns varied spatially and temporally among the three sites. Total phosphorus and K deposition increased significantly at Plastic Lake and decreased significantly at Paint Lake, but there was no significant trend in TP or K deposition at Heney Lake over the 30 year period. All sites, but especially Paint Lake, exhibited considerable inter-annual variation in TP and K deposition. To quantify the contribution of pollen, which represents an internal source of atmospheric P deposition, Durham pollen collectors during the spring and summer of 2014 were used. The three sites, Paint Lake, Heney Lake, and Plastic Lake had pollen deposition amounts of 5202 grains·cm-2, 7415 grains·cm-2, and 12 250 grains·cm-2, respectively in 2014. Approximately 83% of pollen deposition can be attributed to white pine and red pine that has a concentration of 3 mg·g-1 of P. It was estimated that pollen alone could account for up to one-third of annual bulk P deposition. Extrapolating winter P deposition values to exclude all potential biotic influences (insects, bird feces, leaves), indicates that bulk deposition estimates may double actual net P to forests, which has implications for long-term P availability, especially in harvested sites.

Author Keywords: Atmospheric Deposition, Phosphorus, Pine, Pollen, Potassium, South-Central Ontario

The future-poetry of Dennis Lee published in Testament (2012) is the culmination of four cycles of creativity in his lifetime, each seeking a Real beyond the nihilism of technological modernity. Ultimately, Lee wagers the role of the poet and the future of poetic language on Earth on a non-modern that risks entangling the poet who enters void and embodies its meaninglessness.

CHAPTER ONE: To approach this wager, the thesis first identifies the sources in philosophy of a Canadian Romantic modernism embraced by George Grant in collegial exchanges with Dennis Lee during the period of Civil Elegies (1972). Grant elicits a politics out of Nietzsche; Lee extends a poetics out of classical experimental modernism, made intelligible in this thesis by Mallarmé's "cadence" or "rhythm" of things in nothingness and by Beckett's word-play at the impasse of naming. CHAPTER TWO:

To think beyond the mastery of the world by technique is to encounter a choice between silence as assumed by Grant and nonsense as explored by Lee during the period of Alligator Pie (1974) and The Gods (1979). CHAPTER THREE: The example of Paul Celan and his revisiting of Hölderlin provokes Lee to attend upon cadence at the level of the discrete word, an experiment with the dissolution of language and selfhood anticipated in the period of Riffs (1993) and Nightwatch (1996). Here, the undermusic felt

to belong to the life-world (Lebenswelt) impacts as affect, in contrast to Celan's alienated death-walk. CHAPTER FOUR: In the spirit of "post-internet poetry," and by means of the spontaneous polyphonic scoring of cadence, Lee transforms the modernist impasse at the void into a further contradiction, the living of which may allow the poet access to a

non-modern, but at a cost: the loss of poetry to incomprehension and insignificance, the reduction of the poet to a medium of the void, the dissolving of structure into materialities colliding in chance.

Author Keywords: Dennis Lee, George Grant, Nonsense, Paul Celan, Technological Modernity, Void

This thesis is an investigation of the theme of freedom in the work of Gilles Deleuze and Felix Guattari. Chapter One investigates Deleuze and Guattari's concept of becoming as it is articulated in their book A Thousand Plateaus, and seeks to resolve a problem related to their shifting descriptions of the role of agency in the process of becoming, at times described as voluntary, and at other times described as involuntary. We conclude that chapter with a defense of the claim that their shifting descriptions are unproblematic and are, in fact, attempts to illustrate the paradoxical experience of becoming. Chapter Two investigates Deleuze's earlier text, The Logic of Sense, and attempts to make sense of his use of the term destiny. Our conclusion in that chapter is that destiny is neither necessity, pure self-authorship, nor passive resignation, but rather consists of a mixture of activity and passivity, willfulness and chance.

Author Keywords: Agency, Becoming, Counter-actualization, Deleuze and Guattari, Destiny, Freedom

This thesis is a critical response to Evgeny Morozov's article proclaiming the death of the cyberflâneur. Suspicious of the superficiality of his argument, I developed a practico-theoretical project to prove that the cyberflâneur is not dead but alive – or, if it were dead, to rescue it from its grave and bring it back to life. In the course of my response to Morozov, I develop a theoretical foundation that allows me to continue thinking about the concept and practice of the cyberflâneur in the context of the Internet. In doing so, I rely on Gilles Deleuze and Felix Guattari's "Treatise on Nomadology: The War Machine" (2011), in combination with a history of the tradition of wandering. We are living in a postmodern-posthuman era driven by the chaotic and confusing forces that are manifested through the Internet. As such, it is no longer enough to be concerned with opening the space where we live, move and think; we cannot retreat to nature, we can't escape society. However, I see potential in the Internet. The Internet, as a physical and material network, can be actualized as an apparatus of capture. It operates as a medium for accelerating or limiting speed, or as an apparatus for the control of the transmission of information. I develop the cyberflâneur as an aesthetic figure that reveals the Internet's potential. If these revelations happen to be transmitted, then everyday life can again become an object of dispute, rather than unmeditated habituation.

Author Keywords: Cyberflâneur, Everyday Life, Internet, Nomadology, Research-creation, Wandering

Gilles Deleuze claims that understanding the eternal recurrence as a recurrence of the same is a misreading of Friedrich Nietzsche, yet, this assertion is not supported by Nietzsche's texts. In all instances where Nietzsche describes the eternal recurrence, he emphasizes that it is one of the same events. One's willingness to love one's fate and to will the eternal recurrence of the same represents the psychological state of the Overman and his achievement of joyousness. However, this is at odds with Deleuze and Felix Guattari's conception of the nomad.

Consequently, the nomad and the Overman are not congruous at all. Rather, the nomad is Nietzsche's lion. The eternal return of the different then describes the psychological state of the lion as a precursor to the psychological state of the Overman.

The lion cannot will the eternal recurrence of the same; he must will the eternal recurrence of the different. When the lion becomes the child, he has the psychological perspective within which to will the eternal recurrence of the same. It is in this sense that Nietzsche and Deleuze's versions of the eternal recurrence are not antithetical – they are complementary and represent a progression of psychological thought.

Author Keywords: Eternal Recurrence, Friedrich Nietzsche, Gilles Deleuze, Nihilism, Nomad, Overman

This thesis examines a specific figure that appears throughout contemporary Japanese detective fiction (across different media), which I have termed the Shaman detective. A liminal figure that combines Japanese folk cosmologies with contemporary detective work, the Shaman detective is at once similar to, yet separate from, western postmodernist detective fiction. Invested in narratives of enchantment the Shaman detective is marked by his rejection of the epistemological ties of the modern and classical detectives that cause his counterparts to fail in the face of postmodern subjectivism. Committed to il-logic, dreaming, play, and intuition, the Shaman detective exists in the realm of the Fantastic, bridging the gap between mundane and marvellous realities. This thesis reads Shaman detective texts using western postmodernist theory with Todorov's theory of the Fantastic and Jane Bennett's New Materialism. This is synthesized with Japanese thought traditions, cosmologies and philosophies, in order to draw out the Shaman detective.

Author Keywords: Enchantment, Japanese Fiction, New Materialism, Postmodern Fiction, Shamanism, the Fantastic

Northern and southern flying squirrels (Glaucomys sabrinus and G. volans, respectively) are experiencing a climate change induced increase in range overlap, resulting in recent hybridization. We investigated the occurrence of heterospecific communal nesting, a potential facilitator of hybridization, and aimed to confirm the presence of torpor, a potential barrier to hybridization, in flying squirrels. In wild-caught captive squirrels, we conducted a paired nest choice experiment and found that heterospecific nesting did occur, but in a lower frequency than conspecific nesting. Ambient temperature did not affect the frequency of grouped nesting. We attempted to induce torpor in flying squirrels in a laboratory through cold exposure while measuring metabolic rate and body temperature. Strong evidence of torpor was not observed, and metabolic rate remained unchanged with season. We conclude that torpor is not a barrier to hybridization in flying squirrels, but resistance to heterospecific nesting may indicate the existence of one.

Author Keywords: heterospecific group, hybridization, northern flying squirrel, social thermoregulation, southern flying squirrel, torpor