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Graduate Theses & Dissertations
Pages
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- Becoming and Destiny in Deleuze and Guattari
- This thesis is an investigation of the theme of freedom in the work of Gilles Deleuze and Felix Guattari. Chapter One investigates Deleuze and Guattari’s concept of becoming as it is articulated in their book A Thousand Plateaus, and seeks to resolve a problem related to their shifting descriptions of the role of agency in the process of becoming, at times described as voluntary, and at other times described as involuntary. We conclude that chapter with a defense of the claim that their shifting descriptions are unproblematic and are, in fact, attempts to illustrate the paradoxical experience of becoming. Chapter Two investigates Deleuze’s earlier text, The Logic of Sense, and attempts to make sense of his use of the term destiny. Our conclusion in that chapter is that destiny is neither necessity, pure self-authorship, nor passive resignation, but rather consists of a mixture of activity and passivity, willfulness and chance. Author Keywords: Agency, Becoming, Counter-actualization, Deleuze and Guattari, Destiny, Freedom
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- Dennis Lee's Testament
- The future-poetry of Dennis Lee published in Testament (2012) is the culmination of four cycles of creativity in his lifetime, each seeking a Real beyond the nihilism of technological modernity. Ultimately, Lee wagers the role of the poet and the future of poetic language on Earth on a non-modern that risks entangling the poet who enters void and embodies its meaninglessness. CHAPTER ONE: To approach this wager, the thesis first identifies the sources in philosophy of a Canadian Romantic modernism embraced by George Grant in collegial exchanges with Dennis Lee during the period of Civil Elegies (1972). Grant elicits a politics out of Nietzsche; Lee extends a poetics out of classical experimental modernism, made intelligible in this thesis by Mallarmé’s “cadence” or “rhythm” of things in nothingness and by Beckett’s word-play at the impasse of naming. CHAPTER TWO: To think beyond the mastery of the world by technique is to encounter a choice between silence as assumed by Grant and nonsense as explored by Lee during the period of Alligator Pie (1974) and The Gods (1979). CHAPTER THREE: The example of Paul Celan and his revisiting of Hölderlin provokes Lee to attend upon cadence at the level of the discrete word, an experiment with the dissolution of language and selfhood anticipated in the period of Riffs (1993) and Nightwatch (1996). Here, the undermusic felt to belong to the life-world (Lebenswelt) impacts as affect, in contrast to Celan’s alienated death-walk. CHAPTER FOUR: In the spirit of “post-internet poetry,” and by means of the spontaneous polyphonic scoring of cadence, Lee transforms the modernist impasse at the void into a further contradiction, the living of which may allow the poet access to a non-modern, but at a cost: the loss of poetry to incomprehension and insignificance, the reduction of the poet to a medium of the void, the dissolving of structure into materialities colliding in chance. Author Keywords: Dennis Lee, George Grant, Nonsense, Paul Celan, Technological Modernity, Void
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- Denizens of Virtual Worlds
- This thesis studies a subset of players of video games called “power-gamers” who play games in a way that mirrors labour as opposed to leisure. Through ethnographic fieldwork and exploration this thesis examines what constitutes “power-gaming” and seeks to unpack the differences between skill, fun, and labour. Chapter One analyzes how ethnographic fieldwork is performed in virtual worlds, and the necessary frameworks inherent to this. Chapter Two explores facets of technical hobbies, masculinity, skill, and how they relate to power-gaming. Chapter Three explores how different cultures globally choose to play-games, and the forms of sociability involved in this play. Chapter Four examines reality in relation to virtual worlds, and how players in virtual worlds explore and unpack their surroundings, which mirrors many scientific practices in the real world. Chapter Five explores narrative structure in games, and their relation to power-gaming practices. Chapter Six concludes with a discussion of power-gamers as a neo-liberal workforce. Author Keywords: game design, neo-liberalism, playbour, power-gamer, sociability, virtual-ethnography
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- Characterisation of the Giardia Tata-Binding Protein - Preparation for an in vivo approach
- The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a suitable yield and purity and used as the immunogen. The antibody was then tested for reactivity to the native protein in our laboratory. By Western blot analysis, it was possible to observe the enrichment of the gTBP within the nuclear fraction compared to a cytoplasmic fraction extracted from Giardia cells. However, the antibody could not be successfully used in an immunoprecipitation assay - suggesting that the antibody is unable to bind to the native structure of gTBP. Therefore, the focus of this work was changed to analyse gTBP via multiple sequence alignments, homology modelling and BLAST to identify any unique regions that may contribute to its unusual binding characteristics. These techniques were also used to identify specific regions of gTBP that may be used to generate synthetic peptides as immunogens for future antibody production. Author Keywords: ChIP, Giardia intestinalis, Homology modelling, Immunoprecipitation, TATA-binding protein, Western Blotting
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- Regulation of Cytokinins During Kernel Development in High and Low Yielding Oat and Barley Lines
- Cytokinins (CKs) are a family of plant phytohormones responsible for many areas of plant growth and development. There are four free base types of CKs found in higher plants, trans-zeatin (tZ), N6-(∆2-isopentenyl)adenine (iP), cis-Zeatin (cZ) and dihydrozeatin (DZ). CK biosynthesis is regulated by adenosine phosphate-isopentenyltransferase (IPT), which is encoded by a multi-gene family in many plant species. There are two types of IPT pathways responsible for CK production, the tRNA pathway and the AMP (ATP/ADP) pathway. The tRNA pathway putatively produces cZ and the latter predominantly produces iP type nucleotides. CKs have long been studied for their role in stress tolerance, signal transduction, and involvement in many areas of plant growth and development. This study focuses on the role of CKs and CK biosynthesis by IPT during kernel development and comparisons of its regulation in high and low yielding barley and oat lines. The sequence of a putative IPT encoding gene in barley and oat was identified by a blast search of other known IPT gene fragments in closely related species. Quantitative Real time PCR results based on primers designed for the putative barley and oat IPT gene revealed changes in expression of IPT during different stages of kernel development, but no significance difference was associated with yield. Correlation of IPT gene expression in barley with cZ CK profiles measured by HPLC-MS/MS, confirms a putative IPT gene is a tRNA- IPT. HPLC-MS/MS results reveal some CK types, such as benzyladenine, are more predominant in higher yielding lines. This suggests different types of CKs play a role in yield production. Future studies on more IPT genes in the barley and oat IPT gene family will outline a more clear representation of the role of IPT in barley kernel development. Author Keywords: Benzyladenine, Cereal grain, Cytokinin, Isopentenyl Transferase, Mass Spectrometry, Real Time PCR
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- Time-dependent effects of predation risk on stressor reactivity and growth in developing larval anurans (Lithobates pipiens)
- The predator vs. prey dynamic is an omnipresent factor in ecological systems that may drive changes in life history patterns in prey animals through behavioural, morphological, and physiological changes. Predation risk can have profound effects on the life history events of an animal, and is influenced by the neuroendocrine stress response. Activation of the hypothalamic-pituitary-adrenal/interrenal axis, and the induction of stress hormones (e.g., corticosterone (CORT)) have been shown to mediate the onset of inducible anti-predator defensive traits including increased tail-depth, and reduced activity. The predator-prey relationship between dragonfly nymphs and tadpoles can be a powerful model system for understanding mechanisms that facilitate changes in the stress response in accordance with altered severity of risk. It has been well demonstrated early in tadpole ontogeny that increased corticosterone (CORT) levels, observed within three weeks of predator exposure, are correlated with increased tail depth morphology. However, the reactivity of the stress response in relation to the growth modulation in developing prey has yet to be fully explored. Accordingly, this thesis assessed the stress and growth response processes in tadpoles that were continuously exposed to perceived predation risk later in ontogeny. Continuous exposure of prey to predation risk for three weeks significantly increased CORT levels, and tail depth. However, tadpoles exposed to six weeks of predation risk acclimated to the presence of the predator, which was observed as a significant reduction of stressor-induced CORT levels. In addition, although increased tail depth has been attributed to predator defense, predator-naïve tadpoles began to display similar tail depth morphology as treated tadpoles at the six week time point. Thus, this thesis suggests that the stress response in lower vertebrate systems (e.g., tadpoles) may operate in a similarly complex manner to that observed in higher vertebrates (e.g., rats), for which severity of risk associated with the stressor aids in defining activity of the stress response. Moreover, the lack of morphological difference between treatments among tadpoles exposed later in ontogeny suggests that the mechanisms for inducing defenses are normal morphological traits in the development of the animal. This thesis paves the way for future research to elucidate the relationship between the neuroendocrine stress response and hormonal pathways involved in growth modulation in the presence of environmental pressures. Author Keywords: Acclimation, Corticosterone, Growth Modulation, Predation Risk, R. pipiens, Tadpole
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- third wheel
- Population cycles are regular fluctuations in population densities, however, in recent years many cycles have begun to disappear. With Canada lynx this dampening has also been seen with decreasing latitude corresponding to an increase in prey diversity. My study investigates the role of alternate prey on the stability of the lynx-hare cycle by first comparing the functional responses of two sympatric but ecologically distinct predators on a primary and alternate prey. I then populated a three species predator-prey model to investigate the role of alternate prey on population stability. My results showed that alternate prey can promote stability, though they are unlikely to “stop the cycle”. Furthermore, stability offered by alternate prey is contingent on its ability to increase intraspecific competition. My study highlights that population cycles are not governed by a single factor and that future research needs to be cognizant of interactions between alternate prey and intraspecific competition. Author Keywords: alternate prey, Canis latrans, functional response, Lepus americanus, Lynx canadensis, Tamiasciurus hudsonicus
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- Executive Function as a Predictor of Emotional, Behavioural, and Social Competence Problems in Children with Epilepsy
- The study aimed to examine the association between different components of executive function (EF) and emotional, behavioural, and social competence problems (EBSP) in children with epilepsy. Although there is evidence of an association between EBSP and EF in typically developing children, little research has examined this relation in children with epilepsy. The sample comprised of 42 children with epilepsy, aged 6.0 to 18.1 years old. Results showed that EBSP were associated with EF in these children; however, different components of EF were related to different EBSP. Shifting was a significant predictor of emotional, behavioural, and social competence problems in children with epilepsy, whereas inhibition was a significant predictor of behavioural problems. This suggests that children with epilepsy, with different EF profiles may be at-risk for developing different types of problems. These results may aid researchers and clinicians with the development of new techniques to identify and treat children with EBSP. Author Keywords: behavioural problems, emotional problems, epilepsy, executive function, social competence
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- Mutation of the B10 Tyrosine and E11 Leucine in Giardia intestinalis Flavohemoglobin
- The flavohemoglobin in Giardia intestinalis (gFlHb) is the only known protozoan member of a protein class typically associated with detoxifying nitric oxide (by oxidation to nitrate) in bacteria and yeast. Mutants of the B10 tyrosine (Y30F) and E11 leucine (L58A), conserved residues thought to influence ligand binding, were expressed and studied using Resonance Raman (RR) spectroscopy. In the wild type protein, RR conducted using a carbon monoxide probe detects two distinct Fe-CO stretches associated with two different active site configurations. In the open configuration, CO does not interact with any polar side chains, while in the closed configuration, CO strongly interacts with one or more distal residues. Analysis of the Y30F mutant provided direct evidence of this tyrosine’s role in ligand stabilization, as it had only a single Fe-CO stretching mode. This stretching mode was higher in energy than the open conformer of the wild type, indicating a residual hydrogen bonding interaction, likely provided by the E7 glutamine (Q54). In contrast the L58A mutant had no effect on the configurational nature of the enzyme. This was unexpected, as the side chain of L58 sits atop the heme and is thought to regulate the access of distal residues to the heme-bound ligand. The similar spectroscopic properties of wild type and L58A suggest that any such regulation would involve rapid conformational dynamics within the heme pocket. Author Keywords: B10 Tyrosine, Catalytic Globin, E11 Leucine, Flavohemoglobin, gFlHb, Giardia intestinalis
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- Elemental Variation in Daphnia
- Environmental variation can affect consumer trait expression and alter ecological and evolutionary dynamics in natural populations. However, although dietary nutrient content can vary by an order of magnitude in natural ecosystems, intra-specific differences in consumer responses to food quality have not been thoroughly investigated. Therefore, the purpose of my dissertation was to examine the influence of dietary nutrition and other environmental factors on consumer phenotypic variation using the freshwater cladoceran Daphnia. I conducted a series of complementary laboratory and field studies where I examined the effects of dietary phosphorus (P) content and additional biological/environmental variables (multi-elemental limitation, genetic variation, and temperature) on daphnid life-history, biochemistry, body elemental composition, and population growth. In general, phenotypic expression within a species varied significantly in response to all experimental variables, but the relative influence of each was highly context dependent. In my first chapter, I found that dietary P content and environmental calcium (Ca) concentrations both altered Daphnia body Ca:P ratios and growth rates of individuals and affected intrinsic rates of increase at the population level. However, food quality appeared to have a much larger effect on trait expression, and body Ca:P ratios were highly sensitive to other forms of dietary nutrient limitation. Next, I documented significant quantitative genetic variation and phenotypic plasticity in daphnid P content, growth, and P use efficiency of field collected animals grown across dietary P gradients. Trait expression was also influenced by genotype X diet interactions suggesting that consumer responses to dietary nutrient limitation can be heritable and may be adaptive in different nutrient environments. Finally, I found that temperature appeared to override food quality effects and decouple P metabolism in natural Daphnia populations, but total biomass production was affected by both dietary P content and temperature, depending on the nutrient content of the lake. Overall, my dissertation shows that consumer responses to nutrient limitation can vary significantly within a species and that changes in trait expression may be modified by other environmental variables. These results should be incorporated into existing stoichiometric models and used to investigate the eco-evolutionary consequences of consumer phenotypic variation in response to nutritional stress. Author Keywords: ecological stoichiometry, evolution, life-history, nutrient limitation, nutrient metabolism, zooplankton
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- Investigation of a Model for Stain Selection and a Robust Estimation for Area of Origin in Bloodstain Pattern Analysis
- Bloodstain pattern analysis is fundamental to crime scene investigations by providing insight into the estimation of the area of origin of a blood source. The selection of bloodstains from an impact pattern is the foundation from which the estimation of the area of origin is derived. The purpose of this research was to develop a theoretical model for stain selection and establish a more robust estimation for the area of origin in the analysis of impact patterns. This model was developed by using quantitative data from marked zones on the impact pattern target surface. The minimum distance between the known blood source location and a single bloodstain line of trajectory was calculated for every bloodstain within each pattern investigated. It has been determined that the zone location of a bloodstain is relevant for improving accuracy, and that stains with all alpha angles can be used to calculate an area of origin estimation. Finally, research within this thesis also validated directional analysis and investigated the number of stains that may be required for a precise area of origin estimation. Keywords: Forensic science, Bloodstain, Impact pattern, Bloodstain pattern analysis, Statistics, Forensic Physics
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- Politics of Muslim Intellectual Discourse in the West
- The dissertation explores and defends the theory and practice of a Western-Islamic public sphere (which is secular but not secularist and which is Islamic but not Islamist), within which a critical Islamic intellectual universe can unfold, dealing hermeneutically with texts and politically with lived practices, and which, moreover, has to emerge from within the arc of two alternative, conflicting, yet equally dismissive suspicions defined by a view that critical Islam is the new imperial rhetoric of hegemonic orientalism and the opposite view that critical Islam is just fundamentalism camouflaged in liberal rhetoric. The Western-Islamic public sphere offers a third view, arising from ethical commitment to intellectual work, creativity, and imagination as a portal to the open horizons of history. Author Keywords: Critical Islam, critique, history, Islamic reformation, public sphere, secular