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Graduate Theses & Dissertations
Pages
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- Denizens of Virtual Worlds
- This thesis studies a subset of players of video games called “power-gamers” who play games in a way that mirrors labour as opposed to leisure. Through ethnographic fieldwork and exploration this thesis examines what constitutes “power-gaming” and seeks to unpack the differences between skill, fun, and labour. Chapter One analyzes how ethnographic fieldwork is performed in virtual worlds, and the necessary frameworks inherent to this. Chapter Two explores facets of technical hobbies, masculinity, skill, and how they relate to power-gaming. Chapter Three explores how different cultures globally choose to play-games, and the forms of sociability involved in this play. Chapter Four examines reality in relation to virtual worlds, and how players in virtual worlds explore and unpack their surroundings, which mirrors many scientific practices in the real world. Chapter Five explores narrative structure in games, and their relation to power-gaming practices. Chapter Six concludes with a discussion of power-gamers as a neo-liberal workforce. Author Keywords: game design, neo-liberalism, playbour, power-gamer, sociability, virtual-ethnography
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- Phosphorus deposition in forested watersheds
- Phosphorus (P) is an essential macronutrient. In south-central Ontario, foliar P concentrations are low and studies have suggested that P may be limiting forest productivity. Current catchment mass balance estimates however, indicate that P is being retained suggesting that P should not be limiting to tree growth. Phosphorus deposition is measured using bulk deposition collectors, which are continuously open and therefore are subject to contamination by pollen and other biotic material with high P and potassium (K) concentrations and may therefore overestimate net P inputs to forested catchments. Average annual TP and K deposition at three long-term (1984 – 2013) monitoring sites near Dorset, Ontario ranged from 15 to 20 mg·m-2y-1 and 63 to 85 mg·m-2y-1, respectively, and was higher at Paint Lake compared with Plastic Lake and Heney Lake. Phosphorus and K in bulk precipitation were strongly positively correlated, but deposition patterns varied spatially and temporally among the three sites. Total phosphorus and K deposition increased significantly at Plastic Lake and decreased significantly at Paint Lake, but there was no significant trend in TP or K deposition at Heney Lake over the 30 year period. All sites, but especially Paint Lake, exhibited considerable inter-annual variation in TP and K deposition. To quantify the contribution of pollen, which represents an internal source of atmospheric P deposition, Durham pollen collectors during the spring and summer of 2014 were used. The three sites, Paint Lake, Heney Lake, and Plastic Lake had pollen deposition amounts of 5202 grains·cm-2, 7415 grains·cm-2, and 12 250 grains·cm-2, respectively in 2014. Approximately 83% of pollen deposition can be attributed to white pine and red pine that has a concentration of 3 mg·g-1 of P. It was estimated that pollen alone could account for up to one-third of annual bulk P deposition. Extrapolating winter P deposition values to exclude all potential biotic influences (insects, bird feces, leaves), indicates that bulk deposition estimates may double actual net P to forests, which has implications for long-term P availability, especially in harvested sites. Author Keywords: Atmospheric Deposition, Phosphorus, Pine, Pollen, Potassium, South-Central Ontario
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- Characterisation of the Giardia Tata-Binding Protein - Preparation for an in vivo approach
- The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a suitable yield and purity and used as the immunogen. The antibody was then tested for reactivity to the native protein in our laboratory. By Western blot analysis, it was possible to observe the enrichment of the gTBP within the nuclear fraction compared to a cytoplasmic fraction extracted from Giardia cells. However, the antibody could not be successfully used in an immunoprecipitation assay - suggesting that the antibody is unable to bind to the native structure of gTBP. Therefore, the focus of this work was changed to analyse gTBP via multiple sequence alignments, homology modelling and BLAST to identify any unique regions that may contribute to its unusual binding characteristics. These techniques were also used to identify specific regions of gTBP that may be used to generate synthetic peptides as immunogens for future antibody production. Author Keywords: ChIP, Giardia intestinalis, Homology modelling, Immunoprecipitation, TATA-binding protein, Western Blotting
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- Regulation of Cytokinins During Kernel Development in High and Low Yielding Oat and Barley Lines
- Cytokinins (CKs) are a family of plant phytohormones responsible for many areas of plant growth and development. There are four free base types of CKs found in higher plants, trans-zeatin (tZ), N6-(∆2-isopentenyl)adenine (iP), cis-Zeatin (cZ) and dihydrozeatin (DZ). CK biosynthesis is regulated by adenosine phosphate-isopentenyltransferase (IPT), which is encoded by a multi-gene family in many plant species. There are two types of IPT pathways responsible for CK production, the tRNA pathway and the AMP (ATP/ADP) pathway. The tRNA pathway putatively produces cZ and the latter predominantly produces iP type nucleotides. CKs have long been studied for their role in stress tolerance, signal transduction, and involvement in many areas of plant growth and development. This study focuses on the role of CKs and CK biosynthesis by IPT during kernel development and comparisons of its regulation in high and low yielding barley and oat lines. The sequence of a putative IPT encoding gene in barley and oat was identified by a blast search of other known IPT gene fragments in closely related species. Quantitative Real time PCR results based on primers designed for the putative barley and oat IPT gene revealed changes in expression of IPT during different stages of kernel development, but no significance difference was associated with yield. Correlation of IPT gene expression in barley with cZ CK profiles measured by HPLC-MS/MS, confirms a putative IPT gene is a tRNA- IPT. HPLC-MS/MS results reveal some CK types, such as benzyladenine, are more predominant in higher yielding lines. This suggests different types of CKs play a role in yield production. Future studies on more IPT genes in the barley and oat IPT gene family will outline a more clear representation of the role of IPT in barley kernel development. Author Keywords: Benzyladenine, Cereal grain, Cytokinin, Isopentenyl Transferase, Mass Spectrometry, Real Time PCR
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- third wheel
- Population cycles are regular fluctuations in population densities, however, in recent years many cycles have begun to disappear. With Canada lynx this dampening has also been seen with decreasing latitude corresponding to an increase in prey diversity. My study investigates the role of alternate prey on the stability of the lynx-hare cycle by first comparing the functional responses of two sympatric but ecologically distinct predators on a primary and alternate prey. I then populated a three species predator-prey model to investigate the role of alternate prey on population stability. My results showed that alternate prey can promote stability, though they are unlikely to “stop the cycle”. Furthermore, stability offered by alternate prey is contingent on its ability to increase intraspecific competition. My study highlights that population cycles are not governed by a single factor and that future research needs to be cognizant of interactions between alternate prey and intraspecific competition. Author Keywords: alternate prey, Canis latrans, functional response, Lepus americanus, Lynx canadensis, Tamiasciurus hudsonicus
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- Investigation of a Model for Stain Selection and a Robust Estimation for Area of Origin in Bloodstain Pattern Analysis
- Bloodstain pattern analysis is fundamental to crime scene investigations by providing insight into the estimation of the area of origin of a blood source. The selection of bloodstains from an impact pattern is the foundation from which the estimation of the area of origin is derived. The purpose of this research was to develop a theoretical model for stain selection and establish a more robust estimation for the area of origin in the analysis of impact patterns. This model was developed by using quantitative data from marked zones on the impact pattern target surface. The minimum distance between the known blood source location and a single bloodstain line of trajectory was calculated for every bloodstain within each pattern investigated. It has been determined that the zone location of a bloodstain is relevant for improving accuracy, and that stains with all alpha angles can be used to calculate an area of origin estimation. Finally, research within this thesis also validated directional analysis and investigated the number of stains that may be required for a precise area of origin estimation. Keywords: Forensic science, Bloodstain, Impact pattern, Bloodstain pattern analysis, Statistics, Forensic Physics
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- Politics of Muslim Intellectual Discourse in the West
- The dissertation explores and defends the theory and practice of a Western-Islamic public sphere (which is secular but not secularist and which is Islamic but not Islamist), within which a critical Islamic intellectual universe can unfold, dealing hermeneutically with texts and politically with lived practices, and which, moreover, has to emerge from within the arc of two alternative, conflicting, yet equally dismissive suspicions defined by a view that critical Islam is the new imperial rhetoric of hegemonic orientalism and the opposite view that critical Islam is just fundamentalism camouflaged in liberal rhetoric. The Western-Islamic public sphere offers a third view, arising from ethical commitment to intellectual work, creativity, and imagination as a portal to the open horizons of history. Author Keywords: Critical Islam, critique, history, Islamic reformation, public sphere, secular
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- Early Responses of Understory Vegetation to Above Canopy Nitrogen Additions in a Jack Pine Stand in Northern Alberta
- Abstract Early Responses of Understory Vegetation After One Year of Above Canopy Nitrogen Additions in a Jack Pine Stand in Northern Alberta Nicole Melong Nitrogen (N) emissions are expected to increase in western Canada due to oil and gas extraction operations. An increase in N exposure could potentially impact the surrounding boreal forest, which has adapted and thrived under traditionally low N deposition. The majority of N addition studies on forest ecosystems apply N to the forest floor and often exclude the important interaction of the tree canopy. This research consisted of aerial NH4NO3 spray applications (5, 10, 15, 20, 25 kg N ha-1yr-1) by helicopter to a jack pine (Pinus banksiana Lamb.) stand in the Athabasca Oil Sands Region (AOSR) in northern Alberta, Canada. The main objective was to assess the impacts of elevated N after one year of treatment on the chemistry of understory vegetation, which included vascular plants, terricolous lichens, epiphytic lichens and a terricolous moss species. Changes in vegetation chemistry are expected to be early signs of stress and possible N saturation. Increased N availability is also thought to decrease plant secondary compound production because of a tradeoff that exists between growth and plant defense compounds when resources become available. Approximately 60% of applied N reached the ground vegetation in throughfall (TF) and stemflow (SF). Nitrate was the dominant form of N in TF in all treated plots and organic N (ON) was the dominant form of N in SF in all plots. The terricolous non-vascular species were the only understory vegetation that responded to the N treatments as N concentration increased with increased treatment. Foliar chemistry of the measured epiphytic lichens, vascular species, and jack pine was unaffected by the N treatments. Based on biomass measurements and N concentration increases, the non-vascular terricolous species appear to be assimilating the majority of TF N after one year. Vegetation from the high treatment plot (25 kg N ha-1yr-1) was compared to a jack pine forest receiving ambient high levels of N (21 kg N ha-1yr-1) due to its proximity to Syncrude mining activities. Nitrogen concentrations in plant tissues did not differ between the two sites; however, other elements and compounds differed significantly (Ca, Mg, Al, Fe). After one year of experimental N application, there were no environmental impacts consistent with the original N saturation hypothesis. Author Keywords: Athabasca Oil Sands Region, Canopy Interactions, Jack Pine, Nitrogen, Secondary Chemistry, Understory Vegetation
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- Reshaping the Terms of Debate
- The Reagan era instigated a fundamentally conservative shift in the political, economic and discursive climate of America. As Ronald Reagan is a highly divisive symbolic figure in American politics, much of the historiography of his presidency has been characterized by polarized interpretations. Over the past decade there has been a noticeable shift towards more favourable and triumphal interpretations of the Reagan era. This thesis seeks to analyze the ideological shifts that have characterized the trajectory of historical writings on the Reagan era. Through employing a careful textual analysis of key works by Michael Schaller, Gil Troy and Sean Wilentz, amongst others, this study demonstrates how historiography serves us less as an objective means of understanding the past and more so as an expanding collective historical artifact that illustrates the changing currents of intellectual and political discourse. In doing so, the notion of scholarly objectivity itself is thrown into question. Author Keywords: Cold War, Conservatism, Historiography, Neoliberalism, Reagan Doctrine, Ronald Reagan
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- Commonality of Enemies
- Carlism and anarchism were revolutionary social movements that acquired significant popular support during the most intensive period of modernization in Spain (mid 19th to mid 20th centuries). It was noted but not well explored by contemporaries and historians that these enemies were similar in their hostility towards modernization and in their intense idealism. This thesis compares the two movements in order to determine the nature of their commonality and what this suggests about ideological enemies. A range of sources were consulted, including scholarship on modern Spain, biographical information on individuals who converted from Carlism to anarchism and contemporary print media. It was concluded that they were produced by the same destabilizing processes of disentailment and industrialization, which drew the working classes towards proposals that would have otherwise seemed implausibly utopian. The thesis further suggests that they were uniquely idealistic, in that they put moral integrity before the success of their cause. Author Keywords: anarchism, Carlism, enemy other, modernization, Modern Spain, social movements
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- Assessing the Effects of Brief Interventions on Mood and Dream Imagery in Recovering Alcohol and Drug Addicts
- Addiction can lead to a plethora of health, social and economical problems. Substances are used for mood regulation, and therefore, waking day mood is extremely important during recovery of alcohol and drug addiction. The current study examined the effects of Meditation and Sleep Mentation Therapy on mood levels. All participants were male, and currently in treatment programs or early stages of recovery from alcohol and drug addiction. Participants were tested for anxiety and depression and were asked to provide a recent dream, prior and post to participating in the intervention. Dreams were scored using Hall and Van de Castle guidelines for scoring imagery. Results are consistent with previous research in that mood levels changed over the course of the meditation period. Implications for future research are discussed as well as applications of Sleep Mentation Therapy and Meditation in clinical and applied practice. Author Keywords: Addiction, Dream Therapy, Meditation, Mood
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- Habitat use and community structure of grassland birds in southern Ontario agro-ecosystems.
- Most grassland bird populations are in decline, so it is becoming increasingly important to understand how they use agricultural field types and form their communities. I performed point counts in cultural meadow, intensive agriculture, and non-intensive agriculture areas in 2011 and 2012. Generalized linear models were used to determine the habitat relationships of six focal species. I found that non-intensive agriculture was used most often and intensive agriculture was often avoided, but there were exceptions which indicate habitat use can be species-specific. I determined in which habitats competition was likely occurring and which species pairs were competing in 2011. In 2012, I experimentally tested these relationships by introducing artificial competitors onto sites. By comparing presence-absence data from 2011 to 2012, I found evidence of habitat-mediated interspecific and conspecific attraction involving Bobolink and Grasshopper Sparrow. This research contributes to the current understanding of grassland bird community ecology and conservation. Author Keywords: agriculture, BACI, community ecology, habitat use, species at risk, species interactions