Graduate Theses & Dissertations

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Calcium Stress in Daphnia Pulicaria and Exposure to Predator-Derived Cues
In recent decades, declining calcium concentrations have been reported throughout lakes across the southern edge of the Canadian Shield. This raises concern as Daphnia populations have shown to be decreasing as they require calcium not only for survival but to mitigate predation risks. Therefore, the purpose of my thesis was to study the adaptability of Daphnia under calcium limitation and predation risk from Chaoborus. Firstly, I examined the effects of calcium limitation and Chaobours kairomones on daphniid life-history and population growth. I found that low calcium concentrations and Chaoborus kairomones affected Daphnia calcium content, life-history traits, and survival. Next, I focused on how calcium concentrations and Chaoborus abundance affected the calcium content and abundance of daphniids. During this study, I also examined the relationship between the abundance of Daphnia and a competitor Holopedium. I found that calcium concentrations and the abundance of Chaoborus affects daphniid abundance. Overall, results from this study show the importance of considering both predation risk and calcium declines to better determine daphniid losses. Author Keywords: anti-predator responses, Chaoborus, competition , Life-History traits, predator cues, Zooplankton
Characteristics of Models for Representation of Mathematical Structure in Typesetting Applications and the Cognition of Digitally Transcribing Mathematics
The digital typesetting of mathematics can present many challenges to users, especially those of novice to intermediate experience levels. Through a series of experiments, we show that two models used to represent mathematical structure in these typesetting applications, the 1-dimensional structure based model and the 2-dimensional freeform model, cause interference with users' working memory during the process of transcribing mathematical content. This is a notable finding as a connection between working memory and mathematical performance has been established in the literature. Furthermore, we find that elements of these models allow them to handle various types of mathematical notation with different degrees of success. Notably, the 2-dimensional freeform model allows users to insert and manipulate exponents with increased efficiency and reduced cognitive load and working memory interference while the 1-dimensional structure based model allows for handling of the fraction structure with greater efficiency and decreased cognitive load. Author Keywords: mathematical cognition, mathematical software, user experience, working memory
influence of tree species litterfall on soil chemistry and implications for modelling soil recovery from acidification
Decades of acidic deposition have depleted base cation pools in soil over large parts of eastern north America, including the Muskoka-Haliburton region of central Ontario. This region has also experienced a shift in forest species composition over the past 200 years, favouring sugar maple (Acer saccharum Marsh.) at the expense of species such as white pine (Pinus strobus L.) and eastern hemlock (Tsuga canadensis (L.) Carr.). This shift in species composition may have changed soil chemistry over time due to differences in nutrient and metal inputs in litterfall. An analysis of litterfall and soil chemistry was conducted for five tree species commonly found across central Ontario. Stands were established in the Haliburton Forest & Wild Life Reserve and were dominated by one of balsam fir (Abies balsamea (L.) Mill.), eastern hemlock, white pine, sugar maple, or yellow birch (Betula alleghaniensis Britt.). Analysis of mineral soil oxides suggested that these stands were established on similar parent material. Deciduous dominated stands (maple and birch) had greater litterfall mass compared with conifer stands (fir, hemlock, and pine), generally leading to greater macronutrient inputs to the soil. Elemental cycling through the organic horizons was more rapid in deciduous stands, with base cations having the shortest residence times. This suggests that a change from greater conifer dominance to mixed hardwood forests may lead to more rapid elemental cycling and alter the distribution of elements in soil. Forests in the region are typically mixed and the resulting differences in soil chemistry may influence model predictions of soil recovery from acidification. Laboratory leaching tests indicated that both stand type and the acidity of simulated rainwater inputs influenced soil solution chemistry, with deciduous stands generally having a greater buffering capacity than sites dominated by coniferous species. Changes in soil chemistry were examined for each stand type using the Very Simple Dynamic (VSD) biogeochemical model. Simulations showed that soil base saturation began to increase following lows reached around the year 2000, and similar patterns were observed for all stands. When sulphur (S) and nitrogen (N) deposition were held constant at present rates, soil base saturation recovery (toward pre-1900 levels) was marginal by 2100. With additional deposition reductions, further increases in base saturation were minor at all sites. In conjunction with additional deposition reductions, the elimination of future forest harvesting allowed for the greatest potential for recovery in all stands. Overall, these results suggest that changes in forest cover may influence soil chemistry over time, most notably in the organic soil horizons. However, forecasted recovery from acidification is expected to follow similar patterns among stands, since differences in soil chemistry were less significant in the mineral soil horizons which compose a greater proportion of the soil profile. Author Keywords: base cation decline, forest harvesting, litterfall, mineral weathering, soil acidification, VSD model
Short photoperiod lowers visceral adiposity and shifts the molecular clock in Peromyscus leucopus
The length of photoperiod can alter circadian rhythms and change fat depot mass whencombined with environmental temperatures below thermoneutral. To isolate photoperiod effects, we compared the effects of long and short photoperiod exposure at thermoneutrality in photoperiod sensitive, F1 generation adult male white-footed mice (P. leucopus). Mice were housed in long-day or short-day photoperiod conditions at thermoneutrality for 4 weeks. Short photoperiod decreased vWAT mass without changing body weight, scWAT or iBAT mass, or calorie consumption. Short photoperiod increased Adrβ3 and Lpl mRNA expression in vWAT with no change in Ucp1, Pgc1a or Hsl. vWAT Per1, Per2 and Nr1d1 mRNA expression were aligned to the onset of dark and food intake, while Bmal1 and Clock were misaligned. These findings suggest that short photoperiod per se can decrease visceral fat accumulation, without activating thermogenesis, reinforcing that environmental photoperiod should be considered when researching cause and prevention of obesity. Author Keywords: adiposity, circadian rhythm, clock genes, obesity, Peromyscus, photoperiod
Differential expression of cytochrome b5s in Giardia intestinalis during nitrosative stress and encystation
The waterborne protozoan Giardia intestinalis cycles between the environmentally-resistant and infectious cyst and the metabolically-active trophozoite that adheres to the epithelial lining of the small intestine. Adhesion can trigger the innate immune response in epithelial cells, including the synthesis of the free radical nitric oxide (NO) that inhibits cell proliferation and encystation of trophozoites. In this work changes in protein expression of three Giardia isotypes of the redox heme protein cytochrome b5 (gCYTb5 I, II and III) were studied in response to either nitrosative stress or induction of encystation. Two nitrosative stressors, sodium nitrite and the NO donor DETA-NONOate, were used at sub-lethal concentrations (0.5 mM and 0.05 mM, respectively) that do not affect cell proliferation until later time points so that subtle changes in protein expression could be observed in the absence of other confounding factors. Nucleolar gCYTb5-I and nucleoplasmic gCYTb5-III expression patterns were similar in trophozoites exposed to either stressor, showing gradual increases in expression with peaks between 4 and 12 hours, which indicates these cytochromes respond to nitrosative stress and possibly to potential DNA damage in Giardia. In contrast, gCYTb5-II of the peripheral vacuoles, which are part of the endocytic pathway of Giardia, showed little change in expression in response to either stressor. However, changes in gCYTb5-II expression were observed in encysting trophozoites, with a 1.4-fold increase in protein levels at seven hours after induction of encystation, followed by a gradual decrease in expression. These changes are consistent with previous mRNA analysis done in our laboratory and suggest a role for gCYTb5-II in the increase in nutrient uptake during early encystation. Author Keywords: cytochrome, encystation, Giardia, heme, nitrosative, parasite
Assessment of Potential Threats to Eastern Flowering Dogwood (Cornus florida) in Southern Ontario
In Canada, eastern flowering dogwood (Cornus florida L.) is an endangered tree that occurs only in the Carolinian forest of southern Ontario. Threats to this species include habitat fragmentation and the fungal pathogen dogwood anthracnose (Discula destructiva). I conducted a population genetic analysis using seven nuclear microsatellite markers to determine if fragmented populations are genetically isolated from one another and have low levels of genetic diversity. Genetic comparisons suggest on-going dispersal among sites and relatively high genetic diversity within most sites; however, smaller populations and younger trees were less genetically diverse. I also used linear mixed effects models to assess potential relationships between several ecological variables and the prevalence of dogwood anthracnose. Disease severity was higher in trees on shallow slopes and in larger trees; the latter also had higher likelihood of infection. Insights from this study will be important to incorporate into future management strategies. Author Keywords: Cornus florida, Discula destructiva, dogwood anthracnose, Eastern flowering dogwood, endangered, population genetics
Development of a Cross-Platform Solution for Calculating Certified Emission Reduction Credits in Forestry Projects under the Kyoto Protocol of the UNFCCC
This thesis presents an exploration of the requirements for and development of a software tool to calculate Certified Emission Reduction (CERs) credits for afforestation and reforestation projects conducted under the Clean Development Mechanism (CDM). We examine the relevant methodologies and tools to determine what is required to create a software package that can support a wide variety of projects involving a large variety of data and computations. During the requirements gathering, it was determined that the software package developed would need to support the ability to enter and edit equations at runtime. To create the software we used Java for the programming language, an H2 database to store our data, and an XML file to store our configuration settings. Through these choices, we can build a cross-platform software solution for the purpose outlined above. The end result is a versatile software tool through which users can create and customize projects to meet their unique needs as well as utilize the features provided to streamline the management of their CDM projects. Author Keywords: Carbon Emissions, Climate Change, Forests, Java, UNFCCC, XML
Phosphorus delivery in the Rainy-River Lake of the Woods Watershed
Lake of the Woods (LOW) is a large international waterbody which suffers from frequent and widespread algae blooms. Previous studies have highlighted the importance of the lake's largest tributary, the Rainy River (RR) and its significance in total phosphorus (TP) delivery to the LOW. Unfortunately, little is known about TP contributions from the RR and its tributaries within the Canadian portion of the watershed. This thesis examines patterns and sources of TP from four tributaries on the Canadian side of the lower RR region, two of which are predominantly natural, and two that are predominantly agricultural. Relationships between water quality parameters, land use and geologic characteristics were observed over a complete hydrologic year (Oct 1, 2018 - Sept 31, 2019), and through an intensive sampling campaign using a nested watershed approach during the spring high flow and summer low flow periods. Results revealed that TP and total suspended sediment (TSS) concentrations (>100 µg/L and >20 mg/L respectively), and loads (>20 kg/km2 and >3500 kg/km2, respectively), were greater at agricultural sites compared with natural sites (<65 µg/L TP and <15 mg/L TSS concentration, and <20 kg/km2 TP and <4000 kg/km2 TSS export). Total P, TSS, Fe, and Al were significantly positively correlated (R2= 0.26-0.59; p<0.05) and intensive sampling revealed that these relationships were strongest during the spring and at the agricultural sites (R2= 0.73-0.98; p<0.05). In contrast, the summer intensive sampling revealed that TP and redox sensitive Fe were significantly correlated (R2= 0.72; p<0.005), whereas redox insensitive Al and TSS were not, suggesting TP may be sourced via redox processes in the summer due to favourable hydrologic conditions. This was observed not only at sites with high wetland influence, but also at sites with more agricultural presence suggesting that redox sourced TP may also originate from mineral stream bed sediment during low flow periods. This research suggested two primary TP sources in the lower RR region: erosion in the spring, and redox processes (internal release) in the summer. It is recommended that intensive monitoring continue in Canada, and further research be conducted to fully understand the significance of internal P release in the tributaries. Author Keywords: erosion, land use, nutrients, particulates, redox, water quality
Women's Lived Experience of Risk in Pregnancy
Cardiovascular disease (CVD) prevention, treatment and outcomes in women remain largely inequitable globally. Unique sex-specific stages of life, including pregnancy conditions, and their influence on cardiac risk is a growing area of research (Norris et al., 2020). For example, preeclampsia is strongly associated with CVD risk. This connection has led to prevention interventions such as postpartum risk clinics. Research to date on pregnancy and chronic disease is rooted in the medical paradigm of risk and lacks women’s lived experience. The present study qualitatively explored illness and risk perceptions of women with risky pregnancy conditions. Some participants felt self-blame for their conditions. Consequences and severity were focused on “baby first”, while maternal risk was viewed in the distant future. Aspects of the pregnancy experience, including prompt access to mental health support, was viewed as a “blessing in disguise”. Risks, such as lack of agency, and benefits of healthcare risk communication and intervention and implications for practice were also explored. Author Keywords: communication, critical, health care, phenomenology, pregnancy, risk
Educational Data Mining and Modelling on Trent University Students’ Academic Performance
Higher education is important. It enhances both individual and social welfare by improving productivity, life satisfaction, and health outcomes, and by reducing rates of crime. Universities play a critical role in providing that education. Because academic institutions face resource constraints, it is thus important that they deploy resources in support of student success in the most efficient ways possible. To inform that efficient deployment, this research analyzes institutional data reflecting undergraduate student performance to identify predictors of student success measured by GPA, rates of credit accumulation, and graduation rates. Using methods of cluster analysis and machine learning, the analysis yields predictions for the probabilities of individual success. Author Keywords: Educational data mining, Students’ academic performance modelling
Legacy Effects Associated with the World’s Largest Ongoing Liming and Forest Regeneration Program in Sudbury, Ontario, Canada
Soil and tree chemistry were measured across 15 limed sites that were established 14 to 37 years ago within the Sudbury barrens in Ontario, along with two unlimed pre-treatment condition reference sites and an unlimed remnant pine forest. Soil pH and base cation (calcium (Ca), magnesium (Mg), and potassium (K)) concentrations were elevated in surface organic [FH] horizons up to 37-years post limestone treatment. Limestone in the organic horizon was evident by higher Ca/Sr ratios (a good marker of dolomite) in younger sites. Base cation mass budgets were generally unable to account for the mass of added Ca and Mg. Sudbury is characterized by widespread metal contamination. Metal (copper (Cu), nickel (Ni), and lead (Pb)) concentrations were generally greatest within the FH horizon and unrelated to stand age. Copper and Ni concentrations in soil generally decreased with distance from the nearest smelter. Metal partitioning (Kd) in soil was most influenced by soil pH rather than organic matter suggesting that as liming effects fade over time metal availability may increase. Author Keywords: Afforestation, Degraded, Limestone, nutrient, Space-for-time, Sudbury
Sinc-Collocation Difference Methods for Solving the Gross-Pitaevskii Equation
The time-dependent Gross-Pitaevskii Equation, describing the movement of parti- cles in quantum mechanics, may not be solved analytically due to its inherent non- linearity. Hence numerical methods are of importance to approximate the solution. This study develops a discrete scheme in time and space to simulate the solution defined in a finite domain by using the Crank-Nicolson difference method and Sinc Collocation Methods (SCM), respectively. In theory and practice, the time discretiz- ing system decays errors in the second-order of accuracy, and SCMs are decaying errors exponentially. A new SCM with a unique boundary treatment is proposed and compared with the original SCM and other similar numerical techniques in time costs and numerical errors. As a result, the new SCM decays errors faster than the original one. Also, to attain the same accuracy, the new SCM interpolates fewer nodes than the original SCM, which saves computational costs. The new SCM is capable of approximating partial differential equations under different boundary con- ditions, which can be extensively applied in fitting theory. Author Keywords: Crank-Nicolson difference method, Gross-Pitaevskii Equation, Sinc-Collocation methods

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Graduate student theses produced at Trent University. You may also browse theses and dissertations by year, program, or author.

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