Graduate Theses & Dissertations

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It's All in Your Head
The Continuity Hypothesis states that dreams reflect waking day cognition and experiences, which reflect one’s mental health status. As such, dreams are, by extension, cognitions that occur during sleep. To date, Cognitive Behavioural Therapy (CBT) is deemed the most efficacious method of social anxiety treatment by working with cognitions. The current study utilized both CBT and The Storytelling Method (TSM) of dream interpretation, whose methodology is based on CBT; CBT works with waking cognitions and TSM works with sleep cognition. This study examined the effectiveness in decreasing social anxiety symptoms with TSM and comparing its efficacy to a traditional CBT technique. Undergraduate psychology students (N = 36) completed a daily journal of either the TSM or CBT format for two weeks. Participants completed self-report measures of social anxiety, state-anxiety, and depression before and after practicing either method. TSM did not significantly decrease levels of social anxiety, state-anxiety, or depression, whereas CBT significantly decreased only social anxiety levels. Dream content reflecting waking day anxiety and depression did not decrease over time, coinciding with the findings that students did not experience a decrease in their waking day life, supporting the Continuity Hypothesis. Findings suggest anxiety and depression to be very stable in this sample. Future research should explore interventions that are clinician-guided, in a group setting, or, occur over a longer period of time. Author Keywords: Content Analysis, Social anxiety, The Storytelling Method
Expression of Giardia intestinalis flavoenzyme GiOR-1 and characterization of its electron transfer properties
Giardia intestinalis possesses four isotypes of cytochrome b5 (gCYTB-I-IV) that differ from their mammalian counterparts, suggesting different functions in this protozoan parasite. Although the recently discovered Giardia flavoenzyme, GiOR-1, reduces these cytochromes, its properties have not been thoroughly studied, owing to the difficulty in its expression. Here I describe successful conditions for expression of GiOR-1 using autoinduction. GiOR-1 is obtained with flavins bound as indicated by its UV-visible spectrum. Its ability to catalyze electron transfer from donors (NADH, NADPH) to acceptors (oxygen, ferricyanide, cytochrome c, gCYTB5-III) were studied in spectrophotometric rate assays. NADPH is the preferred electron donor, while cytochromes are the preferred electron acceptors. Interestingly, the His-tag used to purify gCYTB5-III decreases its reaction rate with GiOR-1, as an untagged version has slightly faster rates. These findings establish the appropriate conditions for further studies on GiOR-1, including the identification of endogenous electron acceptors. Author Keywords: Autoinduction, Cytochrome b5, Cytochrome P450 oxidoreductase, Giardia intestinalis, GiOR-1, Polyhistidine tag
Responses of Primary Producers and Grazers to Silver Nanoparticle Exposure
The increasing production and use of silver nanoparticles (AgNPs) raise concerns on environmental exposure and impact. A large scale in situ enclosure study was conducted at the Experimental Lakes Area to determine the effect of AgNPs on natural phytoplankton and zooplankton communities. This study investigated AgNPs of varying concentrations (4, 16 and 64 μg/L), dosing regimens (chronic vs. pulse), and capping agents (poly-vinyl pyrrolidone vs. citrate). Phytoplankton communities were influenced only by the natural limnological properties of the system signifying tolerance to AgNPs. Zooplankton community structure significantly changed with AgNP concentration and dosing regimen indicating AgNP sensitivity. A microcosm study investigating the effect of AgNPs and phosphorus-dosed periphyton before and after grazing by two benthic invertebrate species (snails and caddisfly larvae) showed reduced periphyton stoichiometry with AgNP exposure. Grazers foraged less on silver dosed periphyton indicating a preferential choice in food quality. Phosphorus reduced the detrimental effects of AgNPs across all conditions. These studies verify the need for in situ experimental designs to fully investigate the effects of AgNPs and their interaction with environmental factors, multiple species assemblages, and across trophic levels. Author Keywords: benthic invertebrate, Experimental Lakes Area, periphyton, phytoplankton, silver nanoparticles, zooplankton
History and Legacy of the “Orillia Asylum for Idiots
The “Orillia Asylum for Idiots” (1861 - 2009), Canada’s oldest and largest facility for the care and protection of children and adults with disabilities, was once praised as a beacon of humanitarian progress and described as a “community within a community.” Yet, survivors who lived in the facility during the post Second World War period, a time described as the “golden age of children’s rights,” tell harrowing stories of abuse and neglect. Despite the nation’s promise to “put children first” and protect the universal rights of “Canada’s children,” children incarcerated within the Orillia Asylum were subjected to systemic neglect and cultural discrimination, daily humiliation and dehumanization, and physical, sexual, and emotional abuse. Far from being a place for child protection and care, this dissertation finds that the Orillia Asylum was a site of a multi-faceted and all-encompassing violence, a reality that stands in complete contrast to the grand narrative through which the facility has historically been understood. This dissertation considers how such violence against children could occur for so long in a facility maintained by the state, a state invested in protecting children. It finds that children who were admitted to the Orillia Asylum were not considered to be “Canada’s children” at all by virtue of being labelled as “mentally deficient,” “feeble-minded,” “not-quite-human,” and “not-quite-children.” Author Keywords: childhood, disability, Huronia Regional Centre, institutional child abuse, institutional violence, institutionalization
Using the Same Language, but Meaning Different Things
Two dominant narratives emerging throughout the war were the national narrative––that is, the narrative of the war as articulated by the British nation via texts such as political speeches, recruitment posters, and popular music–– and the poetic narrative––that is, the narrative of the war emerging from poets, specifically battlefront poets for the sake of this thesis. One hundred years since World War One, these two narratives are often conceptualized as mutually exclusive, even antithetical to one another. This thesis brings these diverse narratives into conversation with one another by investigating how they both draw on the same rhetorics and yet use these rhetorics to differing ends. Interestingly, the rhetorics employed by both narratives throughout the war endure in contemporary remembrance practices in Britain today. By investigating how each narrative draws on and employs the same rhetorics, this thesis both contextualizes and complexifies contemporary interpretations of contemporary remembrance practices. Author Keywords: Battlefront Poetry , Britain, Narrative, Remembrance, Rhetoric, World War One
WOMEN IN HORROR
The objective of this dissertation is to measure the influence of the contemporary influx of women’s involvement in the horror genre in three dimensional capacities: female representation in horror films, female representation as active, participatory spectators and female representation in the industrial production of horror. Through the combined approach of theoretical and empirical analysis, this dissertation examines the social conditions that facilitated women’s infiltration of the horror genre. Beginning with psychoanalytic theories of spectatorship, it is demonstrated that female filmmakers have challenged horror’s traditional images of victimized women through the development new forms of feminine representation in contemporary horror films. Using data collected from a sample of 52 self-identified female horror fans, it is revealed that the purported invisibility of female horror spectators is a consequence of their alternative modes of consumption. Through interviews conducted with four female producers and an examination of their cultural productions, I illustrate that women have reconstituted the horror genre as a space for inclusivity, political activism and feminist empowerment. Cohesively, these findings reveal the contemporary feminist reclamation of horror to be a form of resistance intended to challenge the patriarchal structures that facilitated women’s historical exclusion from the horror genre. Author Keywords: Abjection, Feminism, Film, Gender, Horror, Psychoanalysis
Re-Living the Residential School Experience
The residential school legacy is one of the darkest chapters in Canadian history. From the mid-1850s to 1996, thousands of Aboriginal children were taken from their homelands and placed in residential schools. Taken against their will, many dreaded attending these schools. Some attended for as long as ten to fifteen years, only to be strangers in their own communities upon their return. In the past thirty years, survivors began disclosing the loneliness, confusion, fear, punishment and humiliation they suffered within these institutions, and also reported traumatic incidents of sexual, physical or emotional abuse. These childhood traumas still haunt them today. This dissertation examines the four compensation processes (Litigation, Alternative Dispute Resolution, Independent Assessment Process and the Common Experience Process) used by survivors to determine whether the compensation payments made to them assisted in reconciliation of their residential school experience. To complete an analysis of the processes, twenty-four residential school survivors from Ontario, Manitoba and British Columbia were interviewed about their experiences with one or more of the compensation processes. The study begins with a historical overview of the residential school legacy and continues with the residential school healing movement that initiated and finalized a negotiated settlement agreement for all living survivors. This dissertation provides a unique perspective to the residential school legacy by using a cultural framework, Anishinabe teachings and concepts to share the voices of residential school survivors. The pivotal Anishinabe teaching within this study comes from The Seventh Fire Prophecy. This prophecy states that: “If the New People will remain strong in their quest, the Waterdrum of the Midewiwin Lodge will again sound its voice.” In this dissertation the residential school survivors are the New People. As the dissertation unfolds the author utilizes various Anishinabe concepts to illustrate how the compensation processes failed to assist the New People to reconcile with their residential school experience. This study presents a medicine wheel understanding of reconciliation and the Residential School Legacy. It concludes with an important message to the second and third generation survivors to continue the reconciliatory efforts that the New People introduced. It is crucial that the children and grandchildren of the New People begin the reconciliation process not only for themselves but for the next seven generations. Author Keywords: Anishinabe, compensation, Indian residential schools, reconciliation, survivors
Nutrient Metabolism of an Aquatic Invertebrate and its Importance to Ecology
Aquatic consumers frequently face nutritional limitation, caused in part, by imbalances between the nutrients supplied by primary producers and the metabolic demands of the consumers. These nutritional imbalances alter many ecological processes including consumer life-history traits, population dynamics, and food web properties. Given the important ecological role of organismal nutrition, there is a need to have precise and specific indicators of nutritional stress in animals. Despite this need, current methods used to study nutrition are unable to distinguish between different types of nutritional limitation. Here I studied nutritional metabolism in the freshwater zooplankter, Daphnia. A greater understanding of nutritional metabolism would allow for the development of dietary bio-indicators that could improve the study of the nutritional ecology of animal consumers. Specifically, I addressed the question: What affects the biochemical composition of a generalist aquatic consumer? My overall hypothesis was that the quantity and quality of the diet affects the biochemical composition in a nutrient specific manner. To test this hypothesis, I examined various response variables involved in nutrient metabolism such as alkaline phosphatase activity, whole metabolome, and free amino acid composition. For each response variable, I grew Daphnia under various nutritional stressors and determined if responses are nutrient specific or are a general stress response. I found the current method of measuring alkaline phosphatase was not a phosphorus specific indicator, as activity increased in all nutrient stressed treatments. Analyzing the whole metabolome resulted in nutritional stressors being separated in multivariate space, with many identified metabolites being significantly different from nutrient rich Daphnia. Upon further examination the daphnids free amino acids profiles are caused by differences between the supply of amino acids from the algae and the demand within the Daphnia. These differences in supply and demand resulted in the ability to classify the nutritional status of Daphnia with the use of discriminant analysis, a classification multivariate model. In addition to a deeper understanding and advanced knowledge of the physiological changes caused by nutrient limitation, this research has provided strong evidence for the application of nutritional biomarkers/profiles to identified the nutritional status of Daphnia. Author Keywords: Bio-indictor, Ecological stoichiometry, Metabolism, Nutritional limitation, Nutritional status
Cemeteries and Hunter-Gatherer Land-Use Patterns
The principle aim of this thesis is to evaluate the applicability of the Goldstein/Kelly hypothesis, which proposes that hunter-gatherer cemeteries emerge as a product of resource competition, and function to confirm and maintain ancestral ties to critical resources. My evaluation centres on a case study of the earliest known cemeteries of the middle Trent Valley, Ontario. To determine whether these predictions are true, I investigated the ecological context of local wetland-based foraging, and undertook a locational analysis to determine if the placement of cemeteries correlates with environmental characteristics that reflect the presence of valuable resources that are unique to these locations. The analysis reveals that ancient cemeteries in the middle Trent Valley were located near seasonal riparian wetlands, possibly to secure wild rice and the variety of fauna it attracts. Through the integration of paleoecological, archaeological, and ethnographic information for the region, this research finds support for the Goldstein/Kelly hypothesis. Author Keywords: Cemeteries, Hunter-Gatherers, Landscape Archaeology, Late Archaic, Middle Woodland, Ontario
It Flows from the Heart
Indigenous Knowledges and intellectual tradition emanate from relationship with land, water, spirit, and the beings of Creation. Knowledge mobilization occurs intergenerationally and through these relationships. The Bodwewaadmii Anishinaabeg have lived in relationship with the Great Lakes since the formation of the lakes. Our stories and practices demonstrate our intimate ties to land, water, and the other than human beings. This dissertation shares some of these practices and stories. Settler colonialism in the Great Lakes has disrupted Bodwewaadmii Anishinaabe relationships and resulted in a diaspora. Following the Federal Indian Removal Act of 1830, individual Bodwewaadmiig and families moved north and inland from the southern shores of Lake Michigan, south to the southern plains of the United States and into Mexico, or seemingly stayed in place in southwestern Michigan. As a result, Bodwewaadmii Anishinaabeg now reside in three colonial nation states—Canada, United States, and Mexico. The disruption of Great Lakes basin-based relationships continues, impacting cultural practices, language, and Knowledges as well as knowledge mobilization. Multilayered settler colonial processes have covered women’s water Knowledges and practices. This dissertation shares narratives of Bodwewaadmii migration, removal and relocation through a lens of disruption and knowledge covering. Returning to ourselves, Biskaabiiyang, is revitalization of culture, language and Knowledges. In addition, Biskaabiiyang is a way of being and a research methodology. This dissertation shares the stories and motivations of over twenty-five Anishinaabe women, men and gender fluid humans working to uncover Knowledges and practices and reweave both into their daily lives, the lives of their grandchildren and their community members. This research builds on historical literature and on a body of literature about cultural practices, water Knowledges, and Indigenous peoples’ relationships with land, water, and the beings of the Great Lakes. It contributes to Indigenous research methodology, Bodwewaadmii Anishinaabe history, and revitalization of language, Knowledges and practices. It has been written in a narrative style and for the benefit of our families and communities. Author Keywords: Anishinaabe Studies, Biskaabiiyang, Indigenous Knowledges, Indigenous Research Methodology, Potawatomi, Water
Electrochemical Biosensors for Neurodegenerative Disease Biomarkers
The onset of neurodegenerative diseases such as Alzheimer’s disease (AD) and amyotrophic lateral sclerosis (ALS) are typically characterised by the aggregation of protein biomarkers into cytotoxic fibrils. Novel means of analysing these biomarkers are needed to expand the literature toward earlier diagnosis of these conditions. Electrochemical sensors could offer the sensitivity and selectivity needed for specialised analysis, including potential point-of-care applications. The AD biomarker Tau, and ALS biomarker TDP-43 proteins are explored here by using a label-free electrochemical sensors. Tau protein was covalently bound to gold electrode surface to study the in vitro mechanisms of aggregation for this protein. An immunosensor to TDP-43 was developed by covalently binding primary TDP-43 antibodies (Abs) on gold electrode surface. A novel direct ELISA sensor for TDP-43 with visual detection and electrochemical quantification was also developed. The results validated the experimental designs toward specialised and selective analysis of these biomarkers and their aggregation mechanisms. Author Keywords: ALS, Alzheimer's, Biosensors, Electrochemistry, Tau, TDP-43
Social Anxiety and Emotional Competence
Prior research has examined social anxiety, emotional competence (EC) and life adjustment (i.e., loneliness and life satisfaction) using cross-sectional designs, although there is limited information on their association over time. The present study examined the impact of social anxiety on life adjustment and assessed if EC could mediate this relationship from young to middle adulthood. University students (N = 283) completed self-report measures at two time points: in first year university and 15 years later. The results accord with previous research demonstrating the stability and slight decrease of social anxiety over time. Social anxiety in young adulthood was a robust predictor of loneliness in middle adulthood, and a weak predictor of life dissatisfaction for men. Mediation analyses revealed that social anxiety was indirectly associated with interpersonal adjustment via EC, especially the intrapersonal EC domain. Social anxiety requires early intervention and EC may help to prevent later social anxiety and maladjustment. Author Keywords: emotional intelligence, life adjustment, social anxiety

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Graduate student theses produced at Trent University. You may also browse theses and dissertations by year, program, or author.

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1973 - 2033
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Format: 2023/10/01

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