Graduate Theses & Dissertations

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From Reading to Reality
This thesis explores post-millennial girl fiction, or young adult works published for girls since the turn of the millennium. Writing for girls has been traditionally placed beneath `more serious' literature, within a hierarchal model, while modern works enjoy an iconic status that is the product of cross-media popularity and a wide readership. Criticism has focused on post-millennial girl fiction being unwholesome, poorly written or anti-feminist, examination of the texts reveals personas which girls may use to explore, rebel against and critically examine societal expectations and fears about girlhood. To explore the publishing phenomenon surrounding current girls' fiction I use two sample series: Gossip Girl by Cecily Von Ziegesar and Twilight by Stephanie Meyer. Chapter One contrasts current girl's fiction with texts written about girlhood, followed with an analysis of the good-girl and bad-girl archetypes which are developed within the two groups of texts. I then consider the stylistic and structural elements presented within the fiction and the impact such elements may have on the girl public. In the conclusion, I consider the wider societal impacts of post-millennial girl fiction through social media, extended readership, cross-media influence and the responses of girl readers. Author Keywords: Feminist Criticism, girlhood, Gossip Girl series, public theory, Twilight series, young adult fiction
Absorbance and Fluorescence Characteristics of Dissolved Organic Matter in North Atlantic, Pacific and Arctic Oceans
This thesis was designed to quantify absorbance and fluorescence characteristics of dissolved organic matter (DOM) in North Atlantic, Pacific and Arctic Oceans. DOM was described in water masses of distinct sources and formation pathways as well as in regions where environmental forcings such as deep water upwelling, enhanced biological activity and receipt of freshwater discharge were prevalent. Influence of sea ice on DOM in Beaufort Sea mixed layer (0 to 30 m) seawater was investigated based on sea ice extent as well as freshwater fractions of meteoric (fmw) and sea ice melt water (fsim) calculated from oxygen isotope ratio (δ18O). The effect of DOM exposure to simulated solar radiation was also assessed to determine the resilience of fluorescent fractions of DOM to photodegradation. This research aims to further our ability to trace DOM in marine environments and better understand its transformation pathways and predict its fate as part of the oceanic carbon cycle in a changing climate. Author Keywords: Absorbance, Arctic Ocean, Dissolved organic matter, Fluorescence, Parallel Factor Analysis, Sea Ice
An Ecological Analysis of Late Woodland Settlement Patterns in the Rouge River Watershed, Southern Ontario
This thesis seeks to understand the influences of environmental variables on site location selection during the Late Woodland period (ca. A.D. 1000-1650) in south-central Ontario, specifically variables considered to be favourable to maize agriculture. Four analyses were undertaken: a geographic information system (GIS) comparative analysis of Late Woodland sites compared to random points; population estimates of four sites for which settlement pattern data was available; maize consumption estimates for these same sites, and; a maize resources catchment analysis of these sites. The analysis conducted did not produce conclusive results to answer questions related to maize-driven site selection, however it did show that requirements for maize resources at these sites could have been met in catchment areas of a 500 m radius, in one case in 250m. The results led to an important question for future research: if agricultural needs were not driving settlement location selection in this area, what was? Author Keywords: Environmental Modeling, GIS, Late Woodland, Maize Agriculture, Movement of Communities, Ontario Archaeology
Flesh Made Real
This thesis examines what the term "transgender narrative" represents at this particular time and location. I do this by examining various methods of transgender storytelling through different forms of media production, including autobiography, film, novels, and online platforms such as Tumblr and YouTube. In chapter one, I look at the production of novels and the value system by which they are judged ("gender capital") in transgender publics and counterpublics. In chapter two, I examine the history of the autobiography, along with the medical history closely associated with transgender identity and bodily transformation. The third chapter examines notions of violence and memorial behind the deaths of transgender people and the ways in which certain political revolutions are formed within a counterpublic. I deconstruct varying notions of identity, authorship, and cultural production and critically examine what it means to be transgender and what it means to tell stories about transgender people. I will conclude with how these stories are being shaped through social media to become more innovative and move away from the rigid value system of gender capital previously mentioned. Author Keywords: autobiography, gender, sex, social media, transgender, transsexual
EVALUATION OF HAYFIELD MANAGEMENT STRATEGIES AND BOBOLINK TERRITORIAL HABITAT IN SOUTHERN ONTARIO
I implemented three hayfield management regimens in southern Ontario (a typical schedule at the farmer`s discretion, a delayed first harvest after July 14, and an early first harvest before June 1 with 65 days before second harvest), and evaluated the costs/benefits to farmers regarding hay quality and feasibility, and to Bobolinks (Dolichonyx oryzivorus) regarding reproductive activity and phenology. Typical management resulted in little to no Bobolink reproductive success, and early harvested sites were not (re)colonized. On delayed harvest sites Bobolinks experienced high reproductive success, but hay quality fell below ideal protein levels for most cattle before harvest. I also examined the habitat features Bobolinks use as the basis for establishing territories and associations between Bobolink territory size and habitat quality. I compared vegetation structure, patch size, and prey abundance between small and large territories. Small territories typically occurred on smaller fields with more preferred vegetation characteristics and greater prey abundance. Author Keywords: agro-ecosystem, Bobolink, Dolichonyx oryzivorus, grassland birds, hayfield management
An Investigation of the Impact of Big Data on Bioinformatics Software
As the generation of genetic data accelerates, Big Data has an increasing impact on the way bioinformatics software is used. The experiments become larger and more complex than originally envisioned by software designers. One way to deal with this problem is to use parallel computing. Using the program Structure as a case study, we investigate ways in which to counteract the challenges created by the growing datasets. We propose an OpenMP and an OpenMP-MPI hybrid parallelization of the MCMC steps, and analyse the performance in various scenarios. The results indicate that the parallelizations produce significant speedups over the serial version in all scenarios tested. This allows for using the available hardware more efficiently, by adapting the program to the parallel architecture. This is important because not only does it reduce the time required to perform existing analyses, but it also opens the door to new analyses, which were previously impractical. Author Keywords: Big Data, HPC, MCMC, parallelization, speedup, Structure
Genome annotation, gene characterization, and the functional analysis of natural antisense transcripts in the fungal plant pathogen Ustilago maydis
Ustilago maydis (DC) Corda is the causal agent of 'common smut of corn'. Completion of the U. maydis lifecycle is dependent on development inside its host, Zea mays. Symptoms of U. maydis infection include chlorosis and the formation of tumours on all aerial corn tissues. Within the tumours, thick-walled diploid teliospores form; these are the reproductive and dispersal agent for the fungus. U. maydis is the model to study basidiomycete biotrophic plant-pathogen interactions. It holds this status in part because of the completely sequenced 20.5 Mb genome; however, thorough genome annotation is required to fully realize the value of this resource. The research presented here improved U. maydis genome annotation through the analysis of cDNA library sequences and comparative genomics. These analyses identified and characterized pathogenesis-related genes, and identified putative meiosis genes. This enabled the use of U. maydis as a model for investigating 'host-induced' meiosis. Further, the cDNA library analyses identified non-coding RNAs (ncRNAs) and natural antisense transcripts (NATs). NATs are endogenous RNA molecules with regions complementary to a protein-coding transcript. Although NATs have been identified in a wide variety of mammals, plants, and fungi, very few have been functionally characterized. Over 200 U. maydis NATs were annotated by analyzing full-length cDNA sequences. NAT structural features were characterized. Strand-specific RT-PCR was used to detect NATs in U. maydis and in a related smut fungus, U. hordei. The data supported a common role for NATs in smut teliospore development, independent of the RNA interference pathway. Analysis of the expression of one U. maydis NAT, as-um02151, in haploid cells, led to a model for NAT function in U. maydis during teliospore dormancy. This model proposed NATs facilitate the maintenance of stored mRNAs through the formation of double-stranded RNA. In testing this model, it was determined that the deletion of two separate upstream regulatory regions, one of which contained a ncRNA (ncRNA1), altered NAT levels and decreased pathogenesis. These studies strengthened U. maydis as a model organism, and began the functional investigation of NATs in U. maydis, which identified a new class of fungal pathogenesis genes. Author Keywords: cDNA library analysis, genome annotation, mRNA stability, natural antisense transcripts, pathogenesis, Ustilago maydis
Abject Utopianism and Psychic Space
This dissertation utilizes the psychoanalytic theories of French psychoanalyst Julia Kristeva as a lens through which to read the novels of American author Samuel R. Delany. I argue that concepts proper to Kristeva's work--namely abjection and/or the abject--can provide a way to think what it might mean to be utopian in the 21st century. Delany's novels are received historically, which is to say his work speaks from a certain historical and cultural viewpoint that is not that of today; however, I claim that his novels are exceptional for their attempts to portray other ways of being in the world. Delany's novels, though, contain bodies, psychologies, and sexualities that are considered abject with respect to contemporary morality. Nonetheless, this dissertation argues that such manifestations of abject lived experience provide the groundwork for the possibility of thinking utopianism differently today. Throughout, what I am working toward is a notion that I call Abject Utopianism: Rather than direct attention toward those sites that closely, yet imperfectly, approximate the ideal, one should commit one's attention to those sights that others avoid, abscond, or turn their nose up at in disgust, for those are the sites of hope for a better world today. Author Keywords: Abject, Delany, Kristeva, Literary Criticism, Psychoanalysis, Utopia
AN EXAMINATION OF THE FUNERARY OFFERINGS PLACED IN MYCENAEAN CHAMBER TOMBS DURING THE PALATIAL AND POSTPALATIAL PERIODS IN THE AEGEAN
Mortuary remains comprise a large part of the archaeological record for the Late Bronze Age in the Aegean. By the Palatial period, chamber tombs became the most common burial type on the Mycenaean Mainland, with their popularity continuing into the Postpalatial period. In addition, a single chamber tomb could be reused for as many as ten generations, resulting in large collections of burials and offerings. On account of the prolific use and reuse of chamber tombs, they provide an abundance of data for studying the mortuary rituals performed by the Mycenaeans during the Palatial and Postpalatial periods. The purpose of this study is three fold: to test the theory that the Mycenaean palatial systems influenced the types of offerings placed in the chamber tombs; to assess the validity of previously stated claims that the offerings placed in the chamber tombs represent funerary rituals, and if so, what type(s) of rituals?; and to establish whether Mycenaean mortuary archaeology is sufficiently well recorded to support a meaningful analysis of variation in funerary depositional patterning. The results of this study provide insight into the nature of the Mycenaean mortuary rituals for chamber tombs. Author Keywords: Chamber Tombs, Late Helladic, Mortuary rituals, Mycenaean, Palatial period, Postpalatial period
Purification and Identification of Selenium-containing C-phycocyanin from Spirulina
Selenium is an essential trace nutrient to many organisms, yet in high concentrations it is toxic. Organic selenium is more bioavailable to aquatic biota than inorganic selenium, but is usually found in much lower concentrations. Algae are known to biotransform inorganic selenium into several organo-selenium compounds, but it is unknown whether any of these bioaccumulate in the food chain. In this study, selenium was incorporated into the methionine residues of an algal photosynthetic protein, c-phycocyanin from Spirulina spp. The extent of selenium incorporation was quantified by inductively coupled plasma-mass spectrometry (ICP-MS), and the protein was identified using electrospray mass spectrometry (ES-MS). C-phycocyanin was isolated and purified from Spirulina with a final recovery of 20-30 % of the total c-phycocyanin present. Selenomethionine replaced 92.8% ± 1.22 of the methionine residues in c-phycocyanin when grown in 2.5 ppm sodium selenite. ES-MS was used to obtain protein spectra, and pure c-phycocyanin was identified. Data of full scans provided estimated masses of both protein subunits--α-chain measured at 18,036 Da; β-chain measured at 19,250 Da--close to the theoretical masses. Protein fragmentation by collision-induced dissociation and electron capture dissociation provided approximately 52 % amino acid sequence match with c-phycocyanin from Spirulina platensis. This study demonstrates the incorporation of selenium into an algal protein, and the identification of c-phycocyanin using electrospray ionization-mass spectrometry. Author Keywords:
THE PROPENSITY TOWARD EXTREMIST MIND-SET AS PREDICTED BY PERSONALITY, MOTIVATION, AND SELF-CONSTRUAL
ABSTRACT The Propensity Toward Extremist Mind-Set as Predicted by Personality, Motivation, and Self-Construal Nick Fauset Multivariate regression analyses were used to determine the effects of Personality (Neuroticism, Extraversion, Openness, Agreeableness, and Conscientiousness), Motivation (External, Amotivation, Intrinsic, and Identified), and Self-Construal (Independent and Interdependent) on three domains of Extremist Mind-Set (Proviolence, Vile World, and Divine Power). Participants consisted of first year undergraduate students (209 females, 76 males) enrolled in Introductory Psychology (N=279) and/or Introductory Economics (N=7), whom participated for course credit. The Motivation measure was problematic for students to complete and this variable was dropped from the model due to missing data. Decreases in Neuroticism, Openness, Agreeableeness, and Interdependent were significantly correlated with increases in Proviolence. Decreases in Agreeableness were correlated with increases in Vile World. Decreases in Openness, and increases in Agreeableness and Interdependent were significantly correlated with increases in Divine Power. These observations provide an interesting perspective on the types of Canadian undergraduate students who are more likely to score highly on measures of Extremism. Keywords: Militant Extremist Mental Mind-Set, Extremism, Personality, Five Factor Model, Motivation, Intrinsic, Extrinsic, Self-Construal, Independent, Interdependent Author Keywords: Extremism, Militant Extremist Mental Mind-Set, Motivation, Personality, Self-Construal
Exploring Indigenous Contributions to (Indigenization of) the City of Saskatoon's 2012-2022 Strategic Plan
The self-determining autonomy of urban Aboriginal communities in Canada's Prairie Provinces can be strengthened at the local scale through decolonized municipal governance frameworks. The City of Saskatoon's Strategic Plan 2012-2022 is highlighted to explore two interrelated questions: do Saskatoon's Aboriginal engagement strategies represent a co-produced or indigenized mainstream planning and policy-making process? Does the potential indigenization of municipal planning and policy-making represent a promising pathway to facilitate local decolonization through collaborative municipal-Aboriginal governance in Saskatoon? Results from qualitative interviews reveal that the City of Saskatoon's distinctive Aboriginal engagement strategies were not entirely meaningful for participants, though the planning process included elements that, if expanded upon, could deepen co-production. Indigenization through co-production necessitates a thorough integration of Aboriginal community input at every stage of a planning and policy-making process, shared control and decision-making mechanisms between municipal governments and Aboriginal communities, and ancillary considerations for increased Aboriginal representation and participation in the administrative and political functions of City Hall. Author Keywords: aboriginal governance, decolonization, indigenization, policy co-production, self-determination, strategic municipal planning

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Graduate student theses produced at Trent University. You may also browse theses and dissertations by year, program, or author.

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