Graduate Theses & Dissertations

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Effects of road salt sodium on soil
While previous studies have focused on how road salt affects water quality and vegetation, limited research has characterized road salt distribution through soil and the resulting impacts. The potential for sodium (Na+) to be retained and impact soil physical and chemical properties is likely to vary depending on the soil’s parent material, and more specifically on the extent of base saturation on the cation exchange complex. This thesis contrasted Na+ retention, impacts, and mobility in roadside soils in two different parent materials within southern Ontario. Soils were sampled (pits and deep cores) during fall 2013 and spring 2014 from two sites along highways within base-poor, Precambrian Shield soil and base-rich soil, respectively. Batch experiments were subsequently performed to investigate the influence of parent material and the effect of co-applied Ca2+-enriched grit on the longevity of Na+ retention in soils. Less Na+ is adsorbed upon the co-application of Ca2+, suggesting grit has a protective effect on soil by increasing cation exchange competition. Positive correlations between Na+ and pH, and negative correlations between Na+ and soil organic matter, % clay and base cations within Shield soils suggest that they are more vulnerable to Na+ impacts than calcareous soils due to less cation exchange competition. However, Na+ is more readily released from calcareous roadside soils, suggesting there is greater potential for Na+ transfer to waterways in regions dominated by calcareous soils. Author Keywords: cation exchange, parent material, road salt, sodium retention, urban soil
Expression optimization and NMR spectroscopy of Giardia intestinalis cytochrome b5 isotype III
The parasitic protist Giardia intestinalis does not synthesize heme and lacks many common eukaryotic heme proteins, yet it expresses four cytochrome b5 (gCYTB5) isotypes of unknown function. These have low reduction potentials and distinct subcellular locations that are consistent with structural features and biological functions that differ from their mammalian counterparts. Isotype III (gCYTB5-III) is particularly fascinating for its unusual location in the nuclei of Giardia. This thesis reports the optimization of recombinant gCYTB5-III overexpression for structural studies by NMR spectroscopy. Vital optimization factors for isotope labelling were first identified, finding that auto-induction promotes the optimization of many other conditions, such as colony selection, starter cultures, media components, temperature, pH and aeration. Optimized conditions were then applied to the expression and NMR spectroscopy of isotope-labelled gCYTB5-III and bovine cytochrome b5 as a control. These results can be extended to other heme proteins and will expand our biochemical knowledge of Giardia. Author Keywords: Auto-induction, Cytochrome b5, Giardia intestinalis, Isotope Labelling, Nuclear Magnetic Resonance Spectroscopy, Recombinant Protein
Entertain Me
The scope of my scholarship has undergone a primarily interdisciplinary approach with an emphasis on historiographic scholarship and method, with the support of communication and sociological theory to underpin my core arguments in each chapter. I use the theories of Third Space, commodified racism, and common sense racism in combination to provide an in-depth analysis of prior scholarship on professional wrestling, contemporary and historic fan activities, and biographic information about professional wrestlers. My first chapter examines prior scholarly methodologies and approaches for broaching the topic of professional wrestling while providing a unique and effective alternative for negotiating with the complex and often-tenuous relationship between professional wrestling, race, and collective memory. I evaluate the seminal works that make up the body of previous professional wrestling scholarship, specifically focusing on dramaturgy as a scholarly approach that limits focus to in-ring performance. In Chapter 2, I provide an in-depth analysis of first-hand accounts by and about Black professional wrestlers, charting the ways in which commodified and common sense racism affect both their careers and personal perspectives on race. Drawing on the tradition of minstrelsy, the chapter defines the ways that Black professional wrestlers have been categorized as mere sources of entertainment rather than being portrayed as skilled, athletic, or serious contenders for wrestling titles. My third chapter assesses twenty-first century fan engagement with professional wrestling content within the context of online Third Spaces. The chapter highlights the points of ideological division amongst fans, who both support and resist the wrestling industry’s common sense and commodifying racism. Keywords: Pro wrestling; commodified racism, common sense racism, Third Space, critical race theory, American history, fan studies Author Keywords: commodified racism, common sense racism, critical race theory, fan studies, Pro wrestling, Third Space
Holocene Resource Exploitation
This study uses the zooarchaeological record to examine the range of activities represented in Late Archaic period samples excavated from Jacob's Island -1B, in the Trent-Severn Waterway region in Ontario. Radiocarbon dates from sixteen features were used to establish a chronology of site use and occupation. The faunal remains analyzed in this study were recovered from seven dated mortuary features associated with human remains. The results of the faunal analysis suggest that Canis lupus familiaris was the primary species interred at Jacob's Island-1B. Small rodents, specifically Tamias striatus were also found in high abundance and are possibly the result of natural burrowing disturbances. Red ochre staining and low levels of burning were identified. Comparisons with other contemporaneous sites in the region indicate some variation in species composition. It is suggested that Canis lupus familiaris was associated with ritual and mortuary activities at Jacob's Island-1B. Author Keywords: Canis lupus familiaris, Late Archaic Period, Ontario, Ritualsim, Zooarchaeology
Local Immigration Partnerships
Introduced as part of the Canada-Ontario Immigration Agreement, Local Immigration Partnerships (LIPs) mark a fundamental shift in local settlement policy. To address the gap in knowledge about the implications of this policy change, this thesis research features a case study of Durham Region's LIP. Objectives were designed to examine the impact of Durham's LIP by interviewing 52 key-informants within six sectors involved in settlement and integration. Findings indicate an effective application of the LIP policy with participants pointing to the LIP's vital role in bringing Welcome Centres to Durham, increasing the attention and profile of immigration issues and improving governance relations amongst different sectors in settlement and integration. A product of local circumstances, the LIP has engaged in a quasi-advocacy role educating mainstream service providers and institutions on how to respond to a diversifying population. Results contribute to the relatively under-studied but growing knowledge of the LIP policy while demonstrating that the localization of immigration policy under the appropriate terms can be successful. Author Keywords: Governance, Integration, Local Immigration Partnerships, Ontario, Regionalization, Settlement
From Reading to Reality
This thesis explores post-millennial girl fiction, or young adult works published for girls since the turn of the millennium. Writing for girls has been traditionally placed beneath `more serious' literature, within a hierarchal model, while modern works enjoy an iconic status that is the product of cross-media popularity and a wide readership. Criticism has focused on post-millennial girl fiction being unwholesome, poorly written or anti-feminist, examination of the texts reveals personas which girls may use to explore, rebel against and critically examine societal expectations and fears about girlhood. To explore the publishing phenomenon surrounding current girls' fiction I use two sample series: Gossip Girl by Cecily Von Ziegesar and Twilight by Stephanie Meyer. Chapter One contrasts current girl's fiction with texts written about girlhood, followed with an analysis of the good-girl and bad-girl archetypes which are developed within the two groups of texts. I then consider the stylistic and structural elements presented within the fiction and the impact such elements may have on the girl public. In the conclusion, I consider the wider societal impacts of post-millennial girl fiction through social media, extended readership, cross-media influence and the responses of girl readers. Author Keywords: Feminist Criticism, girlhood, Gossip Girl series, public theory, Twilight series, young adult fiction
Larval lake whitefish (Coregonus clupeaformis) recruitment dynamics in Lake Huron
Lake whitefish (Coregonus clupeaformis) recruitment has declined substantially in several regions of the Laurentian Great Lakes since the establishment of non-native dreissenid mussels in the early 1990’s. In Lake Huron, the reasons for the observed recruitment declines are currently unknown and there is limited knowledge about larval life stage. In our study, we determined whether larval hatching and growth rates have changed before and after dreissenid mussel invasion, and the role of several key environmental variables in influencing annual variation in larval densities. Larval fish were collected in the Fishing Islands spawning shoal during two time periods: a historical period (1976-1986) and a contemporary period (2017-2019). Larval densities and growth were lower in recent years, suggesting that recruitment is being limited at the larval life stage and that reduced food availability may be further limiting the growth during the larval stage. Annual variation of larval densities were influenced by spawning stock biomass, water levels, and dreissenid mussel presence, with higher water levels and the presence of dreissenid mussels being associated with higher larval densities. The direction of the effect of spawning stock biomass was either negative or positive depending on the model. We also found that larval density was a significant predictor of age 4 recruitment, indicating that year-class strength may be partly established at the larval life stage. Author Keywords: Coregonus clupeaformis, Great Lakes, Lake Huron, Lake whitefish, Larval, Recruitment
Exploring Kiki-Inoomgugaewin
This case study contributes to scholarship surrounding the national conversation on Indigenous language sustainability in North America. Much of this scholarship provides insight on structuring language programs and policies for youth, leaving a tremendous research gap regarding sociolinguistic and cultural research with youth. Youth appear disinterested or otherwise set apart in current research from the development of policies and curriculum concerned with heritage languages. Upon closer inspection; however, youth are engaged and using innovative and different tools than previous generations. This exploration is a foundational case study which builds upon research highlighting the nature of Indigenous language loss in the south as a time sensitive phenomenon as the application of cognitive imperialism and colonial tactics within mainstream schools continue to conceal a large scale cultural and linguistic genocide in Canada. Although Indigenous language loss may seem of concern to only small groups of linguists and dialectic communities, it should in fact concern anyone who cares about reconciliation or closing the tremendous gap in accessing equitable education. The preservation of Indigenous languages and knowledge systems should also be of interest to those parties who seek to comprehensively understand the Natural World and whom have a vested interest in the survival of the planet and protection of the enviroment. Because of these realities, the viewpoints and experiences of all concerned parties are essential. It follows then, that the youth perspective is significant. To address this gap, participatory narrative inquiry was used as a theoretical framework to conduct a foundational case study in which detailed consideration was given to exploring the lived narratives of three Anishnaabeg participants to establish the value of Indigenous youth voice in alternative forms of sociolinguistic and culturally sustainable language learning in the 21st century, and, to strengthen the argument that more research is needed in the field of first-person youth studies. The results of this case study will be useful, specifically, to localized communities of Anishnaabe youth with and for whom much of the research was conducted, and, more generally to youth resistance work focused on media and technology in globalized and contemporary language and cultural ecologies. Research outcomes indicated potential directions for future research in different contexts and localities by presenting commonalities within the fields of social and political engagement and their connection to language and new media in youth populations. It is hoped that this initial material pinpointing a research gap in Indigenous youth language studies will be used to investigate future research in this field. Author Keywords: Anishnaabe, Decolonization, Language, Sociolinguistics, Technology, Youth Studies
Representations of Aboriginal Health in the Media
The goal of the present study was to explore the overall discourse within media articles regarding Aboriginal health issues. The present research aimed to answer the following questions: What Aboriginal health issues are being discussed in the media? How are Aboriginal health issues being discussed in the media? And, does the media propagate power imbalances between Aboriginal and non-Aboriginal Canadians? A thematic analysis was conducted, coopting aspects of Critical Discourse Analysis (CDA) to assess media content. Four CDA devices were used: overlexicalisation, structural oppositions, nominalizations and functional nominations, and concessions and hedging. Results suggest that while there are disparities in health outcomes between Aboriginal and non-Aboriginal Canadians, it is not widely reported in the media. The thematic analysis of 208 articles revealed patterns of stereotypical ideologies and negative framing appearing in media articles, the creation of an us versus them narrative, and themes of out of sight, out of mind, criminalizing Aboriginal Canadians, politicizing health, and access to health services. Author Keywords: Aboriginal Health, Communication, Health, Media, Psychology, Thematic Analysis
An Official Plan for Peterborough, Ontario
Using the Official Plan as the case study, this research examines the extent to which underrepresented groups are engaged in public consultation in the planning process for the City of Peterborough, Ontario, Canada. The Official Plan, along with the tools and secondary plans and policies which reinforce it, shape how people navigate and benefit from the built environment, such as access to public institutions and amenities, transit, parks, safe public space, quality housing, and more. This research frames the Official Plan as an opportunity for the city to demonstrate its new commitment to transparency and community engagement. Drawing on a range of experts and community members, and best engagement practices of other Canadian municipalities and nongovernmental organizations, a set of recommendations is proposed for the city’s community engagement framework. These recommendations emphasize an inclusive, democratic, and feminist approach to engagement and consultation which honours lived experience and local knowledge of diverse and underrepresented demographics and multi-sector stakeholders. Author Keywords: diversity, equity , inclusion, marginalized , public engagement, underrepresented
Detection of four at-risk freshwater pearly mussel species (Bivalvia
Environmental DNA (eDNA) detection uses species-specific markers to screen DNA from bulk samples, such as water, to infer species presence. This study involved the development and testing of species-specific markers for four freshwater pearly mussels (Unionidae). The markers were applied to water samples from intensively sampled mussel monitoring sites to compare species detections from eDNA with established sampling method detections. Target species were detected using eDNA at all sites where they had previously been detected by quadrat sampling. This paired design demonstrated that eDNA detection was at least as sensitive as quadrat sampling and that high species specificity can be achieved even when designing against many sympatric unionids. Detection failures can impede species conservation efforts and occupancy estimates; eDNA sampling could improve our knowledge of species distributions and site occupancy through increased sampling sensitivity and coverage. Author Keywords: conservation genetics, cytochrome oxidase subunit I (COI), environmental DNA (eDNA), quantitative PCR (qPCR), species at risk (SAR)
Size and fluorescence properties of allochthonous dissolved organic matter
Dissolved organic matter (DOM) is a mixture of molecules with dynamic structure and composition that are ubiquitous in aquatic systems. DOM has several important functions in both natural and engineered systems, such as supporting microorganisms, governing the toxicity of metals and other pollutants, and controlling the fate of dissolved carbon. The structure and composition of DOM determine its reactivity, and hence its effectiveness in these ecosystem functions. While the structure, composition, and reactivity of riverine and marine DOM have been previously investigated, those of allochthonous DOM collected prior to exposure to microbes and sunlight have received scant attention. The following dissertation constitutes the first in-depth study of the structure, composition, and reactivity of allochthonous DOM at its point of origin (i.e. leaf leachates, LLDOM), as detected by measuring its size and optical properties. Concomitantly, novel chemometric methods were developed to interpret size-resolved data obtained using asymmetrical flow field-flow fractionation, including spectral deconvolution and the application of machine learning algorithms such as self-organizing maps to fluorescence data using a dataset of more than 1000 fluorescence excitation-emission matrices. The size and fluorescence properties of LLDOM are highly distinct. Indeed, LLDOM was correctly classified as one of 13 species/sources with 92.5% accuracy based on its fluorescence composition, and LLDOM was distinguished from riverine DOM sampled from eight different rivers with 98.3% accuracy. Additionally, both fluorescence and size properties were effective conservative tracers of DOC contribution in pH-controlled mixtures of leaf leachates and riverine DOM over two weeks. However, the structure of LLDOM responded differently to pH changes for leaves/needles from different tree species, and for older needles. Structural changes were non-reversible. Copper-binding strength (log K) differed for the different fluorescent components of DOM in a single allochthonous source by more than an order of magnitude (4.73 compared to 6.11). Biotransformation preferentially removed protein/polyphenol-like fluorescence and altered copper-binding parameters: log K increased from 4.7 to 5.5 for one fluorescent component measured by fluorescence quenching, but decreased from 7.2 to 5.8 for the overall DOM, as measured using voltammetry. The complexing capacity of DOM increased in response to biotransformation for both fluorescent and total DOM. The relationship between fluorescence and size properties was consistent for fresh allochthonous DOM, but differed in aged material. Since the size and fluorescence properties of LLDOM are strikingly different from those of riverine DOM, deeper investigation into transformative pathways and mixing processes is required to elucidate the contribution of riparian plant species to DOM signatures in rivers. Author Keywords: Analytical chemistry, Chemometrics, Dissolved organic matter (DOM), Field-flow fractionation, Fluorescence spectroscopy, Parallel factor analysis (PARAFAC)

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Format: 2024/03/04