Graduate Theses & Dissertations

Pages

Changes in Forms of Uranium in Anoxic Lake Sediments and Porewaters Near an Abandoned Uranium Mine, Bancroft, Ontario
Soluble uranium (U) has been observed continuously in the porewaters of Bentley Lake, a lake with semi-permanent anoxic sediments, despite the fact that reduced U(IV) is known to be insoluble. To be able to predict the fate and mobility of U that has been deposited in lake sediments, it is very important to understand the factors that determine soluble uranium in anoxic environments. Understanding soluble U species is crucial for predicting its behavior in natural systems as well as for the development of U remediation schemes. To explore the factors affecting soluble U in natural environments, anoxic lake sediments and porewaters were tested using two analytic methods, ICP-MS and ESI-HR-MS. Reduced uranium (U(IV)) can be precipitated as U(IV)-NdF3. Using this method revealed that most of the uranium in porewater is not able to be co-precipitated with NdF3. In addition, UO2+ was found using ESI-HR-MS, showing uranyl ions exist in reduced porewater. However, the UO2+ might be attached to some organic groups rather than present as free ions. Seasonal variation and air exposure experiments on the mobility of U between sediments and porewater were observed to test for changes of the redox state of U as a function of sample collection and storage. The results of this study will contribute to better remediation strategies for U tailings and will help U mining operations in the future. Author Keywords:
Changes in the Representations of Women from the 1980s Turkish Cinema to New Turkish Cinema
The thesis examines the changes in the representations of women in Turkish cinema from the 1980s through the 2000s in terms of semiotic codes. To demonstrate the shifts of the representations of women over three decades, four films directed by two representative filmmakers of the 1980s and the 2000s are analyzed within the context of gender codes: A Sip of Love (Atıf Yılmaz/1984), The Night, Angel and Our Gang (Atıf Yılmaz/1994), The Third Page (Zeki Demirkubuz/1999) and Envy (Zeki Demirkubuz/2009). Using gender as code in deconstructing women’s characteristics and their representations in the films, the research highlights the structural image of women in the semiotic sense. The stereotypical representations in Turkish cinema question social norms, patriarchy, and Islamic norms in Turkey. Author Keywords: Gender codes, Islam, Patriarchy, Semiotics, The representations of women , Turkish cinema
Characterisation of the Giardia Tata-Binding Protein - Preparation for an in vivo approach
The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a suitable yield and purity and used as the immunogen. The antibody was then tested for reactivity to the native protein in our laboratory. By Western blot analysis, it was possible to observe the enrichment of the gTBP within the nuclear fraction compared to a cytoplasmic fraction extracted from Giardia cells. However, the antibody could not be successfully used in an immunoprecipitation assay - suggesting that the antibody is unable to bind to the native structure of gTBP. Therefore, the focus of this work was changed to analyse gTBP via multiple sequence alignments, homology modelling and BLAST to identify any unique regions that may contribute to its unusual binding characteristics. These techniques were also used to identify specific regions of gTBP that may be used to generate synthetic peptides as immunogens for future antibody production. Author Keywords: ChIP, Giardia intestinalis, Homology modelling, Immunoprecipitation, TATA-binding protein, Western Blotting
Characteristics of Models for Representation of Mathematical Structure in Typesetting Applications and the Cognition of Digitally Transcribing Mathematics
The digital typesetting of mathematics can present many challenges to users, especially those of novice to intermediate experience levels. Through a series of experiments, we show that two models used to represent mathematical structure in these typesetting applications, the 1-dimensional structure based model and the 2-dimensional freeform model, cause interference with users' working memory during the process of transcribing mathematical content. This is a notable finding as a connection between working memory and mathematical performance has been established in the literature. Furthermore, we find that elements of these models allow them to handle various types of mathematical notation with different degrees of success. Notably, the 2-dimensional freeform model allows users to insert and manipulate exponents with increased efficiency and reduced cognitive load and working memory interference while the 1-dimensional structure based model allows for handling of the fraction structure with greater efficiency and decreased cognitive load. Author Keywords: mathematical cognition, mathematical software, user experience, working memory
Characterizing the demographic history and prion protein gene variation to infer susceptibility to chronic wasting disease in a naïve population of white-tailed deer (Odocoileus virginianus)
Assessments of the adaptive potential of natural populations are essential for understanding and predicting responses to environmental stressors like climate change and infectious disease. The range of stressors species face in a human-dominated landscape, often have contrasting effects. White-tailed deer (Odocoileus virginianus, deer) are expanding in the northern part of their range following decreasing winter severity and increasing forage availability, caused by climate change. Chronic wasting disease (CWD), a prion disease affecting cervids, is likewise expanding and represents a major threat to deer and other cervids We obtained tissue samples from free-ranging deer across their native range in Ontario, Canada which has yet to detect CWD in wild populations of cervids. High throughput sequencing was used to assess neutral genomic variation and variation in the gene responsible for the protein that misfolds into prions when deer contract CWD, known as the PRNP gene. Neutral variation revealed a high number of rare alleles and no population structure, consistent with an expanding population of deer. Functional genetic variation revealed that the frequencies of variants associated to CWD susceptibility and disease progression were evenly distributed across the landscape and the frequencies were consistent with deer populations not infected with CWD. These findings suggest that an observable shift in PRNP allele frequencies likely coincides with the start of a novel CWD epidemic. Sustained surveillance of genomic and genetic variation can be a useful tool for CWD-free regions where deer are managed for ecological and economic benefits. Author Keywords: Canadian wildlife, population genetics, prion, PRNP, RADseq, ungulate
Chemical characterization of dissolved organic matter in relation with hydrography in the Arctic Ocean
In this thesis, water mass distribution of dissolved organic matter (DOM) characteristics (i.e. molecular weight, fluorescent components, thiols and humic substances concentration) was observed in the Arctic Ocean. For the first time, DOM molecular weight (MW) in Beaufort Sea was assessed using asymmetrical flow field-flow fractionation, as well as the first monitoring of thiols and humic substances (HS) using cathodic stripping voltammetry (CSV) in the Arctic Ocean. Based on fluorescence property, DOM characterization was carried out using parallel factor analysis – excitation-emission matrices. Pacific winter waters in the Canada Basin showed higher MW DOM associated with higher fluorescence intensity. High HS was associated with the Arctic outflow waters in top 300 m of the Canadian Arctic Archipelago. Interestingly, maximum thiol concentration was associated with the subsurface chlorophyll-a maximum at most sites, but not universal along the study area. Comparable distributions of CSV-based HS and humic-like fluorescent components suggest similar sources/ processes in the Arctic Ocean. The findings in this thesis suggested DOM characteristics could be used as fingerprints in tracing water masses in the Arctic Ocean. Author Keywords: Asymmetrical flow field-flow fractionation, Cathodic stripping voltammetry, DOM, Metal-binding ligands, Molecular weight, PARAFAC-EEMs
Chew the Fat
Fatty acid analysis was performed on archaeological bone from various fauna from sites in the Canadian arctic to better understand the preservation of fatty acids and their potential applications to palaeoecological and palaeodietary studies. These data were complemented by analyses of modern bone and soft tissue samples from livestock and harp seals (Pagophilus groenlandicus). Results of modern analyses revealed that in terrestrial species, bone has inherently lower concentrations of most fatty acids relative to other soft tissues (adipose, marrow, and muscle). These analyses suggest that the distribution of fatty acids in bone is unique compared to other tissues, and the types and abundances of fatty acids in bone may be linked to dietary sources of lipids. Of the archaeological samples analyzed, terrestrial species (caribou [Rangifer tarandus]) generally exhibited higher concentrations of saturated fatty acids compared to marine species (ringed seals [Pusa hispida] and polar bears [Ursus maritimus]), whereas marine species had higher concentrations of monounsaturated fatty acids compared to terrestrial species. Results of analyses on both modern and archaeological samples provided insight into the degradation of fatty acids in bone, and the rapid loss of polyunsaturated fatty acids in particular. Because the abundances of fatty acids are likely altered in the burial environment, it is recommended that future analyses incorporate compound specific isotope analysis to focus on applications of fatty acids that are typically in the highest abundance and arguably have undergone the least amount of change, including palmitic (C16:0) and stearic acid (C18:0) Author Keywords: Archaeological Science, Bone Lipids, Fatty Acid Analysis, GC-MS, Lipid Preservation, Palaeoecology
Childhood Precursors of Adult Trait Incompleteness
Previous research has suggested that childhood sensory sensitivity may predict adult obsessive compulsive (OC) behaviours. To date, however, research has not addressed how the separate dimensions – harm avoidance and incompleteness - may influence this relationship or why it exists. The current study used a retrospective design to test a) if sensory sensitivity in childhood predicts trait incompleteness in adulthood, as well as b) if emotion regulation variables mediate this relationship. Questionnaires pertaining to OC dimensions and childhood anxieties were completed independently by 172 undergraduate participants and their primary childhood caregiver. Results showed a linear relationship between sensory sensitivity in childhood and incompleteness in adults. Emotion regulation variables failed to mediate this relationship, although a trend for mediation was present. Additionally, exploratory analysis found perfectionism in childhood to be a predictor of trait incompleteness but not harm avoidance, whereas physical anxieties predicted harm avoidance and not incompleteness. Results are discussed in the context of clinical and theoretical implications. Author Keywords: Distress Tolerance, Harm Avoidance, Incompleteness, Obsessive Compulsive Disorder, Sensory Sensitivity, Symmetry
Childhood diet and feeding practices at Apollonia
This study analyses deciduous dental pathology and stable carbon and nitrogen isotopes to investigate the relationship between dietary composition, feeding practices, and oral health in a subadult skeletal sample from the Greek colonial site of Apollonia Pontica, Bulgaria (5th to 3rd century BC). Stable isotope analysis of 74 bone collagen samples indicates that weaning began between the ages of 6 months and 1 year, and was complete by the age of 4. The stable isotope data are consistent with a diet of primarily terrestrial C3 resources. The deciduous dentitions of 85 individuals aged between 8.5 months and 10.5 years were examined for evidence of a number of pathological conditions. The presence of dental caries, calculus, occlusal tooth wear and an abscess indicate that foods introduced early in life affected the oral health of these individuals. Overall, the deciduous dental data correlate well with the stable isotope data and ancient textual sources regarding infant and childhood dietary composition and feeding practices. Author Keywords: breastfeeding, deciduous dentition, dental pathology, stable carbon and nitrogen isotopes, weaning
Civic Agriculture
This thesis re-imagines the social sustainability of civic agriculture. This entails critically examining the idea of sustainability and exposing why a tendency to undertheorize its social dimension is problematic for how we think about sustainability, and consequently for how we do sustainability. What is demonstrated is that we can overcome this tendency by adopting Stephen McKenzie's understanding of social sustainability as a positive condition and/or process within a community. Once brought into contact with the concept of civic agriculture as presented by Thomas A. Lyson, and expanded upon by others, this broadened understanding of social sustainability reveals that we can think of civic agriculture as both a means to, and an expression of, social sustainability. Specifically, this thesis argues that it is civic agriculture's community problem-solving dimension which animates civic agriculture in such a way that it creates the sort of condition and/or enables the sort of process which reflect aspects associated with a substantive and/or procedural understanding of social sustainability. This re-imagining of the social sustainability of civic agriculture provides ways to defend civic agriculture from its critics and is exemplified by drawing from a personal encounter with civic agriculture. In the end, it is proposed that in light of this research there are now good reasons to re-examine civic agriculture and to critically re-imagine what qualifies who as a civic agriculturalist so that the contextual nature of the social sustainability of civic agriculture can be better respected. Author Keywords: civic agriculture, community problem solving, local food systems, social sustainability, Stephen McKenzie, Thomas A. Lyson
Civil Aviation and Scheduled Air Services in Colonial Botswana, 1935-1966
This thesis provides an in-depth and chronological study of the development of civil aviation in the Bechuanaland Protectorate (today’s Botswana), and the role played by the British Government in the development of this form of transport. The thesis argues that Her Majesty’s Government’s neglect and very little interest in its protectorate’s civil aviation represented a form of underdevelopment. The study also reveals the constant contradiction between the neglect of the imperial government and the constant lobbying on the part of colonial administration in the Protectorate for the establishment of an air service. To the colonial administrators, civil aviation represented a symbol of modernity and progress as well as more practical advantages such as mobility. The thesis finally concludes that the Bechuanaland Protectorate’s first airline was established due to growing nationalism both locally and on the continent, at large. The British Government facilitated the establishment of the airline as an attempt to appear benevolent to the protectorate on the eve of independence. Author Keywords:
Class Struggle and Solidarity in Neo-Liberal Times
The lengthy and raucous 1986 Gainers meatpacking plant strike in Edmonton, Alberta was one of the most important events in recent Alberta labour history. In the midst of the economic crisis of the 1980s and the rise of neo-liberal ideas, the strike marked a backlash by both the labour movement and ordinary citizens against attacks on workers and unions. Characterized by widely covered picket line violence, repressive police and court actions, and government unresponsiveness, the strike generated massive solidarity within and beyond the labour movement. This solidarity originated in a rejection of the neo-liberal new reality of Alberta typified by high unemployment, anti-union laws and practices, and lack of government welfare support, and it generated a provincial change the law campaign, national boycott, and rising class consciousness. The working class mobilization during the Gainers strike was a watershed for the Alberta labour movement. Author Keywords: Alberta Federation of Labour, Gainers strike, neo-liberalism, solidarity, working class

Pages

Description

Graduate student theses produced at Trent University. You may also browse theses and dissertations by year, program, or author.

In collections

Search Our Digital Collections

Query

Enabled Filters

  • (-) ≠ Reid
  • (-) ≠ Doctor of Philosophy
  • (-) ≠ Business education

Filter Results

Date

1973 - 2033
(decades)
Specify date range: Show
Format: 2023/09/24

Collection

Author Name

Show more

Name (Any)

Show more

Degree

Degree Discipline

Show more

Subject (Topic)

Show more