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Expression and characterization of cytochrome b5 from Giardia lamblia
Giardia lamblia is an intestinal parasite found globally in freshwater systems that is responsible for endemic outbreaks of infectious diarrhea. As a unicellular parasite that lacks mitochondria, a respiratory chain and lives in the anaerobic environment of its host's intestine, Giardia was assumed for decades to lack heme proteins. However, its genome encodes several putative heme proteins, including three with sequence similarity to the cytochrome b5 family, referred to as Giardia cytochromes b5 (gCYTb5). Recombinant expression of one of these genes (gCYTb5-I), results in a protein (17-kDa) that is isolated with noncovalently bound heme. Resonance Raman and UV-visible spectra of gCYTb5-I in oxidized and reduced states resemble those of microsomal cytochrome b5, while sequence alignment and homology modelling supports a structure in which a pair of invariant histidine residues act as axial ligands to the heme iron. The reduction potential of gCYTb5-I measured by cyclic voltammetry is -165 mV vs the standard hydrogen electrode and is relatively low compared to those of other family members. The amino- and carboxy-terminal sequences that flank the central heme-binding core of the gCYTb5 are highly charged and do not occur in other family members. An 11-kDa core gCYTb5-I variant lacking these flanking sequences was also able to bind heme; however, we observe very poor expression of this truncated protein as compared to the full-length protein. Author Keywords: b-type cytochrome, cytochrome b5, electron transfer protein, Giardia intestinalis, heme/heam protein, spectroelectrochemistry
AN INTEGRATED APPROACH TO WASTEWATER MANAGEMENT AND REUSE IN JORDAN
This research explores the obstacles Jordan is facing regarding the sustainable treatment and reuse of wastewater in the agricultural sector. It assesses the technical, socio-cultural, and political aspects of decision-making around water and wastewater management in Jordan by focusing on a case study involving wastewater usage in the Jordan Valley. It includes a literature review and interviews with representatives of key stakeholders. While at one level wastewater treatment is a technical process with technological solutions, a nuanced understanding of the non-technical challenges facing the wastewater treatment sector in Jordan is necessary. These challenges are inherently embedded in and contextualized by a series of historical, complex and dynamic political and socio-cultural issues involving stakeholders at local and national levels. Only through an interdisciplinary approach with real stakeholder engagement will meaningful solutions to these challenges be developed and implemented, and at least a portion of Jordan's water needs be meaningfully addressed. Author Keywords: Agriculture, Jordan Valley, Political challenges, Sociocultural challenges, Technical challenges, wastewater management
Soil Geochemistry and Normative Mineralogy across Canada
Soils play a crucial role in ecosystem functioning, for example, soil minerals provide important provisioning and regulate ecosystem services. This study used major soil oxides from the North American Soil Geochemical Landscapes Project (n=560) to assess elemental associations and infer soil minerals through exploratory data analysis and to determined quantitative soil mineralogy using a normative method, Analysis to Mineralogy (n=1170). Results showed elemental variability of oxides across the provinces of Canada and strong correlations occurred between elements indicative of soil mineral composition (e.g., Silicon and Aluminium). Principal component analysis inferred soil minerals from soil oxides trends on biplots and classified minerals, generally, as carbonates, silicates, and weathered secondary oxides. Spatial variability in minerals (quartz, plagioclase, potassium feldspar, chlorite, and muscovite) was related to the underlying bedrock geology. The use of Analysis to Mineralogy led to a reliable method of quantifying soil minerals at a large scale. Author Keywords: Analysis to Mineralogy, Exploratory data analysis, Normative procedures, North American Soil Geochemical Landscapes Project, Soil geochemistry, Soil mineralogy
Effect of SP600125 JNK Inhibitor on Cadmium-Treated Mouse Embryo Forelimb Bud Cells In Vitro
This study investigated the role of the JNK signaling pathway in cadmium-treated mouse embryo forelimb bud cells in vitro. Primary cultures of forelimb bud cells harvested at day 11 of gestation were pre-treated with JNK inhibitor SP600125, and incubated with or without CdCl2 for 15, 30, 60, 120 minutes and 24, 48 hours or 5 days. Endpoints of toxicity were measured through cell differentiation by Alcian Blue Assay and phosphorylation of JNK proteins by Western blot. The results demonstrated that, in the cell differentiation assay, inhibiting JNK activation by 20 μM SP600125 causes an enhanced toxic effect in limb cells and inhibits cell differentiation, whereas 2 μM decreases differentiated nodule numbers under both cadmium stress and normal conditions. In conclusion, the JNK pathway has an essential role in the differentiation processes of limb bud cells in normal growth conditions. Author Keywords: Cadmium, Cell Signaling, JNK, Limbs, Mouse Embryo, Teratology
Cytokinin biosynthesis, signaling and translocation during the formation of tumors in the Ustilago maydis-Zea mays pathosystem
Cytokinins (CKs) are hormones that promote cell division. During the formation of tumors in the Ustilago maydis-Zea mays pathosystem, the levels of CKs are elevated. Although CK levels are increased, the origins of these CKs have not been determined and it is unclear as to whether they promote the formation of tumors. To determine this, we measured the CK levels, identified CK biosynthetic genes as well as CK signaling genes and measured the transcript levels during pathogenesis. By correlating the transcript levels to the CK levels, our results suggest that increased biosynthesis and signaling of CKs occur in both organisms. The increase in CK biosynthesis by the pathosystem could lead to an increase in CK signaling via CK translocation and promote tumor formation. Taken together, these suggest that CK biosynthesis, signaling and translocation play a significant role during the formation of tumors in the Ustilago maydis-Zea mays pathosystem. Author Keywords: Biosynthesis, Cytokinins, Signaling, Translocation, Ustilago maydis, Zea mays
Calving site selection and fidelity in a restored elk (Cervus elaphus) herd in Bancroft, Ontario, Canada
ABSTRACT Calving site selection and fidelity in a restored elk (Cervus elaphus) herd in Bancroft Ontario, Canada. Michael R. Allan Parturition site selection by ungulates is believed to be influenced by forage abundance and concealment from predators. In 2011 and 2012, I used vaginal implant transmitters and movements to identify calving sites for 23 GPS collared elk (Cervus elaphus) from a restored herd. I tested the hypothesis that maternal elk used sites with higher forage and denser concealment compared to pre-calving sites at micro and macrohabitat levels. I detected no significant microhabitat differences from direct measurements of vegetation. At the macrohabitat scale, based on proximity of landcover classes, mean distances to hardwood forests was significantly less for calving (153 m) than pre-calving sites (198 m). Site fidelity is hypothesized to offer security in terms of familiarity to an area. I tested the hypothesis that females demonstrated fidelity to their previous year's location during pre-partum, parturition, post partum, breeding and winter periods. Elk were more philopatric during parturition and post partum than during breeding. Compared to winter elk were more philopatric during pre-partum, parturition and post-partum periods. Expressed as distance between consecutive-year calving locations, site fidelity varied with 27% of females exhibiting high (<1 km), 18% moderate and 55% (>2.9 km) low fidelity. I measured nearest-neighbour distances at calving time, exploring the hypothesis that females distance themselves from conspecifics. Elk increased the average distances to collared conspecifics during parturition; however, sample sizes were small. This strategy might influence calving site selection. Rapid movement prior to parturition, low site fidelity and spacing-out of females during parturition appear to be strategies to minimize predator risk and detection. Little evidence of selection for vegetation structure suggests this may not be limiting to these elk. Author Keywords: calving, elk, fidelity, movement, parturition, selection
Can Shared Platforms Build Sustainability in the Non-profit Sector? Comparing Practitioner Perceptions of Organizational Experience in Non-profit Organizations and Platform Projects
This thesis explores practitioner perceptions of operational and structural experiences of non-profit organizations (NPO) and platform projects (PP) to develop an understanding of how a shared platform governance model can build resiliency and sustainability in non-profit organizations. The objectives of this research are to 1) develop an understanding of NPO and PP operational and structural experiences based on qualitative interviews with practitioners; 2) analyze how a PP model can address challenges facing the NPO sector; and 3) based on a thorough analysis of current literature and research findings, recommend a model that addresses these challenges and builds sustainability in NPOs. A grounded inductive approach was used to identify a thematic narrative. The process was iterative, working between existing literature and interview data. Interviews with eight NPO practitioners and eight PP practitioners revealed four narrative theme areas: Financial, Funder, Organizational, and Emotional Tone. The results document several ways a PP model can provide opportunities to address the perceived funder and organizational challenges of small NPOs. Keywords: non-profit organizations, collaboration, innovation, governance models, shared platform, platform projects Author Keywords: governance model , innovation , non-profit organizations , organizational resilience, shared platform, Sustainability
Cluster Approach Applied to the One-Dimensional Anderson-Hubbard Model
S. Johri and R. Bhatt developed a real-space renormalization group approach aimed at extracting the localized single-particle eigenstates of the Anderson model from a large system by identifying clusters of resonant site potentials. E. Campbell generalized this real-space renormalization group approach using standard perturbation theory. Both approaches were intended to approximate the single-particle density of states of the Anderson model. In this thesis, we aimed to test the potential of applying a similar real-space renormalization group approach to calculate the density of states of the interacting Anderson-Hubbard model. Our interest in the density of states of this model is due to a V-shaped zero-bias anomaly in two-dimensional systems. A real-space renormalization group approach is best applied to a one-dimensional system. We found that the zero-bias anomaly is not V-shaped in one-dimension. To test the potential of a real-space renormalization group approach, we used the cluster approach which is the same as the non-interacting renormalization group approach but without the perturbation theory and found that for strong disorder this technique could accurately calculate the density of states over a wide range of energies but deviated from exact results at the band edge, at $\omega=\pm U$ and near $\omega=0$. The first two inaccuracies will be reduced with a proper real-space renormalization group approach. We suspect that the last inaccuracy is associated with long range physics and may be difficult to recover. We also developed a technique that adjusts the identification of clusters in the cluster approach to improve the computation time of the density of states with minimal loss of accuracy in a tunable range around the Fermi level. We found that this technique significantly reduced the computation time and was able to preserve the density of states near the Fermi level, except at the smallest energies near $\omega=0$. Author Keywords: Anderson-Hubbard model, renormalization group, Strong electron correlations, Zero-bias anomaly
Landscape and its Discontents - Art and Ruins, a Critical Topography in Word and Image
From Altdorfer and Poussin to Cézanne, Monet and to the Group of Seven, landscape has been a focal point of artistic inspiration for most of what we think of as modern art history. In contemporary times the concept and representation of landscape has shifted from visions of an idealized and exalted place to notions of the landscape as a ruins and site of ecological disaster. Because of this seismic inversion, artists are no longer solely making visual the beauty and serenity of nature but are rather finding novel ways of problematizing it and incorporating themes of its eventual disappearance, its inescapable transformation into ruins. The following dissertation puts forward a critical topographical study of three sites and three different artists who deal with this new found relationship to landscape. The three landscapes are located in different parts of the world and from different artistic contexts yet showing that they retain an aesthetic and conceptual character that links them together is part of the work of the dissertation. The first site is El Sol del Membrillo, a film by Víctor Erice in which the filmmaker chronicles painter Antonio Lopez García’s attempts to paint the ephemeral, he attempts to paint that which is in the act of disappearing. The second site is The Mill St Cemetery in Cambridge, England where artist Gordon Young has contributed a work of public art titled Bird Stones that blurs the line between landscape, sculpture, monument and artwork. The third and final site is Tommy Thompson Park in Toronto, Canada that presents itself as an ecology park of retrieval, recovery and as a public art space. My investigation of this last regional research site is offered both as a chapter and as a videography about wilderness as wasteland. Author Keywords: Aesthetics, Anthropocene, Art, Cinema, El Sol del Membrillo, Toronto: The Leslie Spit
Critical Topographies of two films
The following thesis is a work in Critical Topography that choses as its site of study two documentary films. The films being studied are El Sol del Membrillo by Victor Erice and Rivers and Tides by Thomas Riedelsheimer. My approach to critical topography in the thesis is twofold: first, I have traced the topical motifs that have appeared to me as I looked at the two films; second, I have translated the films into writing –with the purpose of creating a sourcebook for my analysis- thus bounding the visual content of the films into the delineated space of the written word. I have sought in my analysis to make visible the numerous conceptual, aesthetic, and philosophical notions that are repeated in each film. These notions include materiality, formal operations, temporality, memory, and failure. All of which are ideas that find expression - despite their significant differences - in both documentary films. Author Keywords: Art, Critical Topography, Film Studies, Land Art, Painting, Time
Engaging the Unwritten Text
This study is an attempt to look at how orality plays a role in modern society to move people to action in a social engineering process. By examining the theories for the formation of publics as outlined by Jurgen Habermas and Michael Warner, I argue for the existence of an oral public and further show that it can be engineered with some of the tools provided. This theoretical foundation provides a pathway for a thorough examination of orality as a tool for social engineering and shows how the practices moved the people in the past. In this study, I posit that the oral traditions are still alive and well in modern times and still function as a tool for moving people to social action. To achieve this, orality makes use of popular culture. This study examines elements of popular culture with a view to unearthing the presence of oral modes and how they are still carrying on the same function of social engineering in a modern society. This study concludes by positioning orality as a relevant tool for social engineering in modern Nigerian society and affirms that it is still relevant in the areas of politics, literature and cultural productions with possibilities yet untapped in the area of digital technology. Author Keywords: Nigeria, Orality, Popular culture, Publics, Public Sphere, Social Engineering
Laughing to be Citizens
This study will focus on how immigrants from Sub Saharan African (SSA) countries use humour as a tool for integration and belonging (and ultimately citizenship) in Canada. My aim is to investigate, through a detailed analysis of popular culture productions from immigrant communities, the strategies and techniques of humour that immigrants employ as a mode of communication with fellow immigrants, their immediate host community and the governmental authorities of Canada. I am particularly interested in how African immigrants use their oral background and cultural memory in the production of jokes and other humour products as a way of interacting, first with fellow immigrants as the primary audience and recipients of the humour and, second, with Canadian society at large. Using the ‘Signifying’ theory of Henry Louis Gates (1988) and Mikhail Bakhtin’s (1968) concept of the “Carnivalesque” as the theoretical framework for this study, I argue that immigrants from SSA countries are using humour to question hegemonic regulations that portrays them as victims, while providing alternative narratives of themselves as subjects with human agency. I further postulate that immigrants are taking advantage of the policy of multiculturalism that exists in Canada in a positive manner as an enabler for their humour. In turn, they are using the humour produced to communicate and break down social barriers, while building bridges across communities and social strata. I bolster my arguments with a consideration of humour in three genres of popular culture – literature, standup comedy and film – to show how immigrants rely on their home culture to produce humour in an effort to find belonging in Canada as contributors rather than victims. This thesis is the first work to examine SSA humour, produced by immigrants from these countries, in the context of their immigration and integration into Canada, and the first to present extended literary criticism of the works of immigrant writers, Tololwa Mollel, Yabome Gilpin-Jackson and Segun Akinlolu. This is also the first study on the comedy of Arthur Simeon, originally from Uganda and the film of Phina Brooks, originally from Nigeria. My analysis apprehends the immigrant voice in the writings and productions of these artists and places their works in conversation with Canadian literary/cultural criticism. Until now, there has been no study of the function of humour produced by African immigrants in Canada. It is my hope that this study will not only fill that gap, but also lay the groundwork for future study in this field that I believe holds a lot of socio-cultural promise, especially in the area of cohesive habitation amongst different ethnic groups. This study aims to contribute to conversations on immigration and its impact on Canadian society as part of nation-building and national consciousness. Author Keywords: African Stand up Comedy, Humour, Immigration, Multiculturalism, Popular Culture, Postcolonialism

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