Graduate Theses & Dissertations

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Mfsd8 regulates growth and multicellular development in Dictyostelium discoideum
The neuronal ceroid lipofuscinoses (NCLs), commonly known as Batten disease, are a family of inherited neurodegenerative lysosomal storage disorders. CLN7 disease is a subtype of NCL that is caused by mutations in the MFSD8 gene. MFSD8 encodes a lysosomal transmembrane protein that is predicted to play a role in transporting small substrates across membranes. However, little is known about its role and substrate specificity. Previous work identified an ortholog of human MFSD8 in the social amoeba Dictyostelium discoideum and reported its localization to endocytic compartments. In this study, the effects of mfsd8 loss during Dictyostelium growth and multicellular development were further characterized. Dictyostelium mfsd8- cells displayed increased rates of proliferation and pinocytosis in liquid media. During growth, loss of mfsd8 altered lysosomal enzymatic activities and reduced the intracellular and extracellular levels of autocrine proliferation repressor A. mfsd8- cells grown on a lawn of bacteria formed plaques in a shorter period of time compared to WT cells, providing additional support for the enhanced growth of mfsd8- cells. Upon starvation, the aggregation of mfsd8- cells was delayed, and mfsd8- cells formed more mounds that were smaller in size, which may be attributed to the reduced cell-substrate adhesion and altered lysosomal enzymatic activities observed for mfsd8- cells. Following aggregation, tipped mound formation was delayed, however, loss of mfsd8 did not affect the timing of slug/finger and fruiting body formation. Additionally, slug migration was reduced in mfsd8- cells. These aberrant phenotypes, excluding fruiting body formation, were effectively or partially rescued when Mfsd8-GFP was introduced into mfsd8- cells. Overall, these results show that Mfsd8 plays a role in regulating growth and developmental processes in Dictyostelium via lysosomal-associated functions. Author Keywords: CLN7, Dictyostelium discoideum, Lysosomes, MFSD8, Neuronal Ceroid Lipofuscinoses
Breach, Digital Disruption, the Event
The goal of this dissertation is to formulate a critique of embodiment through the lens of Lacanian psychoanalysis and in the context of digital media and intelligent technologies. Along with psychoanalysis, our research methodology and philosophy are informed by Badiou’s philosophy of the event, Stiegler’s philosophy of technics and infrastructuralism in media studies. Our study aims to uncover and articulate the implicit conditioning by the primordial trauma constitutive of the subject’s encounters with the medium. In our analysis of embodiment, we make extensive use of psychoanalytic concepts such as: the breach, the lack, the signifier, the construction of the object, the object-a, the transitional object, fetishism and phobia. Each of the four parts of the dissertation approaches the topic from a specific angle, such as the theoretical critique of the basic concepts of the subject and object, the function of the metaphor in the context of intelligent technologies, the role of technological infrastructure in the embodiment of the trauma, the construction of a transitional object such as a pop song in the context of technological dystopianism. We demonstrate that the cognitivist presumption concerning the pivotal role of intellectual motivation behind the subjective attachment to information technologies can be significantly shaken from the point of view of the psychoanalytic understanding of drives and jouissance. On the basis of Alain Badiou’s argument, we show the limitations of structurally-determined forms of embodiment considered by psychoanalysis and draw a dividing line between them and the more rare cases of the subjectivation by events suggested by Badiou’s theory. We discuss Badiou’s concept of the work of truth as an advanced alternative to the psychoanalytic concept of the object and the paradigm of the construction of the (transitional) object. We distinguish the Badiouian concept of the event from the idea of technological/digital disruption, on the one hand, and correlate the latter with the problems discussed in psychoanalysis such as the foreclosure of the Name-of-the-Father and the crisis of the paternal metaphor along with the problems of the futurity and the disruption of intergenerational communication discussed by Bernard Stiegler on the other. Author Keywords: Badiou, digital media, Lacan, metaphor, transitional object, work of truth
Governance as If Our Lives Depended On It
This research explores how the value of sustaining the natural world could become foundational to senior level policy decisions in Canada and how Indigenous Knowledges and Peoples could play a key role in such a paradigm shift. It is a trans-disciplinary study that draws on scholarship in Indigenous Studies, Sustainability Studies and Public Policy and existing report recommendations and policy documents that highlight both historical and recent governance trends in the area of sustainability. These sources help to describe both the challenges and the art of the possible in achieving a policy paradigm shift in Canada. The focal point is a series of conversations with seventeen highly experienced Indigenous and non-indigenous policy leaders from across Canada and across traditional territories. The findings reveal that many participants strongly agreed that a paradigm shift should occur and that both Indigenous and western worldviews are needed to realize it, with none disagreeing. They also point to significant changes that are needed to move from paradigms where shorter-term economic development decisions take precedence over environmental concerns to understanding that a healthy economy and society are dependent upon the natural world. To this end, they provide recommendations such as embedding the Right to a Clean Environment in federal legislation and learning from consensus and culturally based governance models in the North West Territories, Nunavut and New Zealand. They suggest mandating education and awareness programs for civil servants and elected officials on Indigenous -Canada relations and sustaining the natural world upon which Canada is situated and upon which treaties are based. They emphasize that a culture shift requires more Indigenous Peoples in senior leadership roles and to be more meaningfully involved in policy processes. Overall, the conclusion finds that a paradigm shift requires positive relationships between parliamentary governments and Indigenous peoples that enable both Knowledge Systems to come together to put the natural world at the foundation of senior-level policy decisions. Qualities such as respect, listening, trust, reciprocity, responsibility and connectedness with the natural world are highlighted through real-world examples that show that, although it may take time, a paradigm shift is possible and may have already begun. Next steps suggest new approaches for building relationships into the policy cycle. Author Keywords: Governance, Policy, Sustainability, Indigenous Knowledges, Natural World

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Graduate student theses produced at Trent University. You may also browse theses and dissertations by year, program, or author.

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1973 - 2033
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