Graduate Theses & Dissertations

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Functional Variation within Middle Paleolithic Ground Stone Tools
In the southern Levant, ground stone tools (GST) provide insight into early plant food exploitation, butchery, and cognition. Outside of these examples, GST evidence is scarce, particularly for the Middle Paleolithic. An extensive assemblage of GST recovered from Nesher Ramla, an open-air hunting camp in Israel, presents the unique opportunity to study the role of GST within Middle Paleolithic behaviour. Use-wear and residue analysis, together with replication experiments are employed to investigate GST function within a specific period of site use by focusing on GST from the Upper Sequence (Units I-II) which reflects a trend of decreasing site-use intensity. The results indicate that GST were employed for bone breaking and knapping during the final phases of occupation while comparison with Unit V suggests longer occupations involved more diverse and extensive use of GST. GST at open-air sites are also proposed to represent a strategy for intensive exploitation of location-specific resources. Author Keywords: Ground Stone Tools, Hammerstones, Middle Paleolithic, Residue Analysis, southern Levant, Use-Wear Analysis
Fungal pathogen emergence
The emergence of fungal hybrid pathogens threatens sustainable crop production worldwide. To investigate hybridization, the related smut fungi, Ustilago maydis and Sporisorium reilianum, were selected because they infect a common host (Zea mays), can hybridize, and tools are available for their analysis. Hybrid dikaryons exhibited filamentous growth on plates but reduced virulence and limited colonization in Z. mays. Select virulence genes in the hybrid had similar transcript levels on plates and altered levels during infection of Z. mays relative to each parental dikaryon. Virulence genes were constitutively expressed in the hybrid to determine if its pathogenic development could be influenced. Little impact was observed in hybrids with increased expression of effectors known to modify host response and metabolism. However, increased expression of transcriptional regulators of stage specific pathogenic development increased the hybrid’s capacity to induce symptoms. These results establish a base for investigating molecular aspects of fungal hybrid pathogen emergence. Author Keywords: effectors, hybrid pathogenesis assays, Sporisorium reilianum, transcription factors, Ustilago maydis, virulence factors
Gene flow directionality and functional genetic variation among Ontario, Canada Ursus americanus populations.
Rapidly changing landscapes introduce challenges for wildlife management, particularly for large mammal populations with long generation times and extensive spatial requirements. Understanding how these populations interact with heterogeneous landscapes aids in predicting responses to further environmental change. In this thesis, I profile American black bears using microsatellite loci and pooled whole-genome sequencing. These data characterize gene flow directionality and functional genetic variation to understand patterns of dispersal and local adaptation; processes key to understanding vulnerability to environmental change. I show dispersal is positively density-dependent, male biased, and influenced by food productivity gradients suggestive of source-sink dynamics. Genomic comparison of bears inhabiting different climate and forest zones identified variation in genes related to the cellular response to starvation and cold. My thesis demonstrates source-sink dynamics and local adaption in black bears. Population management must balance dispersal to sustain declining populations against the risk of maladaptation under future scenarios of environmental change. Author Keywords: American black bear, Dispersal, Functional Genetic Variation, Gene Flow Directionality, Genomics, Local Adaptation
Genetic Networks to Investigate Structure and Connectivity of Caribou at Multiple Spatial and Temporal Scales
Understanding genetic structure, connectivity, and movement of a species iscritical to management and conservation. Genetic network approaches allow the analysis of genetic information with flexibility and few prior assumptions. In chapter one, I tested the ability of individual-based genetic networks to detect fine-scale structure and connectivity in relation to sampling efforts. My findings revealed individual-based genetic networks can detect fine-scale genetic structure of caribou when using 15 highly variable microsatellite loci. Sampling levels less than 50% of the estimated population size resulted in highly disconnected networks which did not allow for accurate structure analysis; however community detection algorithms were robust in grouping closely related individuals despite low sampling. In chapter two, I used individual-based and population-based genetic networks to investigate structure, connectivity, and movement of caribou across a large study area in Western Canada. A community detection algorithm partitioned the population-based genetic network at multiple spatial scales which uncovered patterns of hierarchical genetic structure and highlighted patterns of gene flow. The hierarchical population structure results aligned with the known distribution of different caribou Designatable Units (DUs) and additional structure was found within each DU. Furthermore, individual-based networks that were constructed with a subset of samples from the Mackenzie Mountains region of the Northwest Territories revealed patterns of long-distance movement and high connectivity across the region. Author Keywords: Biological Conservation, Caribou, Community Detection, Connectivity, Genetic Networks, Structure
Genetic diversity and differentiation of Ontario’s recolonizing fishers (Pekania pennanti)
Fishers (Pekania pennanti) were extirpated from many parts of Ontario in the early 20th century, but as of the early 2000s the species had recolonized most of its historical range. While the primary population genetic structure of fishers in central and eastern Ontario has not changed drastically over the past ten years, we did find evidence of increased secondary structure and a reduction in northward movement from southeastern Ontario, a site of recent immigration from the Adirondacks in northern New York. This may be indicative of a reduction in density and thus in density-dependent migration, or it may be a consequence of the population reaching equilibrium following a period of rapid expansion associated with recolonization. We also observed no variation within central and eastern Ontario at 14 of 15 candidate functional loci we screened, suggesting possible directional or stabilizing selection and a lack of adaptive potential. Author Keywords: fisher, functional genes, Ontario, Pekania pennanti, population genetics, recolonization
Genomic architecture of artificially and sexually selected traits in white-tailed deer (Odocoileus virginianus)
Understanding the complex genomic architecture underlying quantitative traits can provide valuable insight for the conservation and management of wildlife. Despite improvements in sequencing technologies, few empirical studies have identified quantitative trait loci (QTL) via whole genome sequencing in free-ranging mammal populations outside a few well-studied systems. This thesis uses high-depth whole genome pooled re-sequencing to characterize the molecular basis of the natural variation observed in two sexually selected, heritable traits in white-tailed deer (Odocoileus virginianus, WTD). Specifically, sampled individuals representing the phenotypic extremes from an island population of WTD for antler and body size traits. Our results showed a largely homogenous genome between extreme phenotypes for each trait, with many highly differentiated regions throughout the genome, indicative of a quantitative model for polygenic traits. We identified and validated several potential QTL of putatively small-to-moderate effect for each trait, and discuss the potential for real-world application to conservation and management. Author Keywords: evolution, extreme phenotypes, genetics, genomics, quantitative traits, sexual selection
Geochemistry and Toxicity of a Large Slag Pile and its Drainage Complex in Sudbury, Ontario
This study was designed to determine the geochemistry and potential toxicity of water draining a large slag pile in Sudbury, Ontario, which runs through a pond complex prior to entering Alice Lake. Slag leaching experiments confirmed slag is a source of sulphate, heavy metals (including Fe, Al, Ni, Co, Cu, Zn, Pb, Cr, Mn) and base cations (Ca, K, Mg, Na). Concentrations of most metals draining through slag in column experiments were similar to metal concentrations measured at the base of the slag pile, although base cations, S and pH were much higher, possibly because of water inputs interacting with the surrounding basic glaciolacustrine landscape. The increase in pH rapidly precipitates metals leading to high accumulation in the surface sediments. Away from the base of the pile, an increase in vegetation cover leads to an increase in DOC and nutrients and transport of metals with strong binding affinities (Cu). Total metal concentration in water and sediment exceed provincial water quality guidelines, particularly near the slag pile, however WHAM7 modeling indicated that the free metal ion concentration in water is very low. Nevertheless, toxicity experiments showed that water with greater concentrations of solutes collected close to the slag pile negatively impacts D. magna suggesting that water draining the slag pile can adversely impact biota in nearby drainage areas. Author Keywords: geochemistry, heavy metals, leaching, non-ferrous slag, precipitation, toxicity
Gratitude is in our nature
Although a wealth of research supports nature’s beneficial effects on well-being, a lack of attentiveness and appreciation for nature may prevent people from fully experiencing nature’s benefits. A mindfulness-based nature intervention was tested across two studies to investigate whether gratitude and mindfulness can boost nature’s effects for well-being. Students completed activities across a one-week (Study 1; N = 129) and two-week (Study 2; N = 130) intervention. Students in both studies experienced significant declines in positive and negative emotion over time. Study 1 did not show the expected increases in gratitude and connection to nature. Study 2 found that students who simply spent time in nature experienced fewer negative emotions and greater increases in trait gratitude than those who were asked to gratefully reflect on their natural surroundings. Both studies found positive correlations between trait gratitude, mindfulness, well- being and connection to nature. Implications and suggestions for future research are discussed. Author Keywords: Gratitude, Intervention, Mental Heath, Mindfulness, Nature, Well-being
Green Leadership in the Classroom
Concerns about climate change means that there is an urgent need to understand teachers’ role in educating students about environmental issues and sustainability. However, little is known about teachers’ environmental leadership and how that affects their competencies in the classroom, their general well-being and connections with nature, or what kinds of personality characteristics shape these teachers. A sample of current, future, and past Canadian teachers (N = 260) completed an online survey which included quantitative and qualitative questionnaires. Correlational and regression analyses determined teachers who possess environmental leadership qualities have a greater connection with nature, more positive well-being, and are more confident in their abilities to teach students outdoors. Furthermore, positive personality traits predict teachers’ environmental leadership. Qualitative data revealed both structural and psychological barriers reduced the likelihood of teachers taking students outdoors and that greater support, resources and training are needed to enable teachers to implement more nature-based learning. Author Keywords: competence, environmental leadership, nature relatedness, personality, teachers, well-being
Ground-truthing effective population size estimators using long-term population data from inland salmonid populations
Effective population size (Ne) is a foundational concept in conservation biology, in part due to its relationship to the adaptive potential of populations. Although Ne is often estimated for wild populations, it is rarely calibrated against actual population estimates (Nc) other than to produce Ne/Nc ratios. This project used demographic and genetic data for from two intensively-studied populations of lake trout (Salvelinus namaycush) in Ontario’s Experimental Lake Area (ELA) as baseline data for evaluating the performance of multiple Ne estimators. Several temporal and single-time (point) genetic methods of estimating Ne were compared against demographic Ne estimates and known population data, as well as variation and consistency within and among Ne estimators. Changes in genetic Ne estimates over time were also compared to changes in demographic structure and fluctuating census estimates, including the effect of an experimentally manipulated population bottleneck on demographic and genetic Ne estimates during population reduction and recovery. Sampling years that included the most pre-, during and post-bottleneck data revealed the lowest estimates using temporal estimators (Ne = 16 to 18) despite pre- and post-bottleneck census estimates of 591 and 565. Estimation of Ne had increasingly tighter confidence intervals as sample sizes approached the actual number of breeding individuals in each population. Performance differences among the tested estimators highlight their potential biases and reliance on different assumptions, illustrating their potential value and caveats for assessing adaptive potential of wild populations. Author Keywords: Effective Population Size, Experimental Lakes Area, Fish Population Assessment, Lake Trout, Population Demographics, Population Genetics
Groundwater Recharge in a Managed Forest on the Oak Ridges Moraine, Southern Ontario
Groundwater recharge was estimated and compared in two open grasslands, three mixed deciduous forest stands (100+ years in age), three young red pine plantations (27 ¨C 29 years in age) and two old red pine plantations (62 ¨C 63 years in age) on the Oak Ridges Moraine, southern Ontario, Canada. Recharge was estimated using a 1-d water balance with measured precipitation, throughfall, stemflow, snowpack water equivalent and soil water storage, and modelled evapotranspiration. Throughfall distribution beneath red pine canopies showed no consistent variation with distance from the tree boles. Old red pines were not major stemflow producers and although the young red pines showed a slight tendency to focus stemflow (focussing ratio > 1), the inclusion of focussed stemflow when calculating recharge at the stand scale made little difference. Conversely, sugar maple (the predominant species in the mixed deciduous stands) showed a strong tendency to focus throughfall proximal to tree boles and produce large quantities of stemflow, resulting in relatively high soil moisture contents and enhanced opportunities for recharge within ~ 0.5 m of tree boles. Inclusion of these focussed inputs resulted in a ~ 11 ¨C 18 % increase in stand scale recharge estimates. The interpretation of land cover control on recharge was complicated by variations in soil texture between sites. Soil texture and its influence on soil water storage capacity resulted in temporal variations in recharge, with sites exhibiting large storage capacities producing less recharge in the fall and greater recharge in the spring than sites with limited storage capacities. Recharge estimates for the entire study period or seasonal values for sites grouped on the basis of soil water storage capacities showed a general trend of increasing recharge in the order: old red pine ¡Ö young red pine ¡ú mixed deciduous forest ¡Ö open grasslands. The disparity between the red pine plantations and the other sites was driven in large part by greater modelled evapotranspiration in the red pine plantations. The similarity in recharge between mixed deciduous forests and open grasslands was the result of focused inputs and less soil evaporation and transpiration in the mixed deciduous forests compared to the open grasslands. The results of this study suggest planting red pine on grasslands on the Oak Ridges Moraine will initially decrease recharge and this decrease will continue as the red pines mature. However, as the red pine plantations are succeeded by mixed hardwood stands recharge will recover to that of the initial grasslands. Author Keywords: Groundwater Recharge, Land Cover Type, Oak Ridges Moraine, Stemflow, Throughfall, Water Balance
HABITAT SELECTION AND LIFE-HISTORY TRAITS OF BREEDING BIRDS IN THE BOREAL-TUNDRA ECOTONE, WITH SPECIAL ATTENTION TO THE AMERICAN ROBIN (TURDUS MIGRATORIUS)
I investigated biodiversity of birds and vegetation associations along the boreal-tundra ecotone in Ivvavik National Park, Yukon Territory, and breeding adaptations used by American Robins (Turdus migratorius) at high latitudes. Twenty bird species were detected over three years using point-count surveys. Densities of American Robin, Dark-eyed Juncos (Junco hyemalis), and Yellow-rumped Warbler (Dendroica coronata) had positive relationships with tree and shrub density, whereas density of White-crowned Sparrows (Zonotrichia leucophrys) was negatively related to tree density. American Robins at this latitude raised only one brood, but females laid slightly larger clutches, the young fledged earlier, and pairs experienced higher nest-success than American Robins at more southerly latitudes. American Robins selected nest sites with high vegetation volume, at both the nest-site, and the nest-patch. This study is important for the first description of the bird community at this high latitude location, and describing how a species at the northern limit of the boreal forest has adapted to living with short-breeding seasons. Author Keywords: American Robin, Ivvavik National Park, Life History, Nest-stie selection, Northern limit

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Graduate student theses produced at Trent University. You may also browse theses and dissertations by year, program, or author.

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1973 - 2033
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