Graduate Theses & Dissertations

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Retrograde Amnesia of Fear Memories Following Pentylenetetrazol Kindling
Memories pertaining to fearful events are some of the most salient and long-lasting memories, as they are critical to the survival of an organism. Seizures induce aberrant changes within temporal lobe and limbic brain structures that are critical for supporting fear memories. Seizures can occur at any time; therefore, it is imperative that research address how seizures impact previously learned information. The present series of experiments demonstrate that pentylenetetrazol-kindling induces retention deficits of previously acquired context fear memories in male rats. Kindling induced subsequent fear learning deficits but did not impact spatial learning. Additionally, following kindling, volumetric increase was observed within the hippocampal subfield CA3, as well as increased neural activation within the hippocampal subfield CA1. The results of this work suggests that chronic seizures can alter the function of neural networks important for supporting and retrieving previously acquired memories. Author Keywords: amygdala, anterograde amnesia, context fear conditioning, hippocampus, retrograde amnesia, seizures
Utilizing Class-Specific Thresholds Discovered by Outlier Detection
We investigated if the performance of selected supervised machine-learning techniques could be improved by combining univariate outlier-detection techniques and machine-learning methods. We developed a framework to discover class-specific thresholds in class probability estimates using univariate outlier detection and proposed two novel techniques to utilize these class-specific thresholds. These proposed techniques were applied to various data sets and the results were evaluated. Our experimental results suggest that some of our techniques may improve recall in the base learner. Additional results suggest that one technique may produce higher accuracy and precision than AdaBoost.M1, while another may produce higher recall. Finally, our results suggest that we can achieve higher accuracy, precision, or recall when AdaBoost.M1 fails to produce higher metric values than the base learner. Author Keywords: AdaBoost, Boosting, Classification, Class-Specific Thresholds, Machine Learning, Outliers
Balance is key
While preferences for symmetry are seemingly universal, they can be seen at their most extreme among individuals high in trait incompleteness. As yet, it is unclear why incompleteness yields heightened symmetry preferences. Summerfeldt et al. (2015) speculated that individuals high in incompleteness may develop heightened preferences for symmetry due to its greater perceptual fluency. Accordingly, the aim of the present set of three experiments was to examine this relationship. Implicit preferences for symmetry were measured using a modified version of the Implicit Association Test (IAT) reported by Makin et al. (2012). Experiments 1 (N = 24) and 2 (N = 24) examined whether the general implicit preferences for symmetry and influence of perceptual fluency reported by Makin et al. (2012) extended to a within-subjects design. Experiment 3 (N = 86) examined whether trait incompleteness is related to greater implicit preferences for symmetric stimuli, and whether perceptual fluency affects this association. Results showed that incompleteness and implicit preferences were related, and that incompleteness-related differences in preferences were eliminated when the patterns were equally perceptually fluent, supporting the idea that incompleteness-related preferences for symmetry are linked to perceptual fluency. Implications of these findings are discussed. Author Keywords:
Thinking High When Feeling Shy
Social anxiety often co-occurs with substance use disorders – various psychological variables, contextual factors, and implicit cognitions may help explain their relationship. This thesis examined whether social anxiety and psychological variables (jointly and independently) helped predict substance use and related problems. It also explored whether social anxiety group membership helped predict implicit cannabis-sedation associations and substance use desirability. A sample of undergraduate student volunteers (N = 65) completed a computer task, questionnaires with anxiety-provoking vignettes, and online questionnaires. Results indicate that fear of negative evaluation and anxiety sensitivity are important predictors of alcohol and cannabis (respectively) use and problems. Social anxiety group was related to increased cannabis desirability in performance contexts. No significant implicit cannabis-sedation associations were identified. Our findings highlight the importance of certain variables in social anxiety and substance use relationships, and considering contextual factors when assessing substance desirability. It also provides preliminary evidence of a novel implicit cannabis-sedation measure. Author Keywords: Coping, Emotion, Implicit Cognition, Social Anxiety, Substance Use
Enhancing post-mortem interval estimates
The growth of immature insects that develop on human remains can be used to estimate a post-mortem interval (PMI). PMI estimate confidence is negatively affected by: larval killing and preservation methods altering their size, limited morphological parameters to assess larval growth and therefore age, and few available alternate species development data. I compared live specimens to preserved specimens of the same development stages to assess the effects of killing-preservation techniques on morphology, and I introduce a new method that uses digital photography to examine maggot mouthparts for stage grading of Phormia regina. Digital photographic methods enable live insects to be quantified and improve approximations of physiological age. I then use these digital methods to produce a growth-rate model for a beetle commonly found on human remains, Necrodes surinamensis, providing data for PMI estimates that was previously unavailable. Author Keywords: Forensic Entomology, Insect development, Morphometrics, Necrodes surinamensis, Phormia regina, Postmortem interval
Asserting sexual (dis)interest
Sexual assertiveness encompasses skills in refusing unwanted sexual situations and bringing about wanted sexual situations. Measures of sexual assertiveness typically assess both refusal and initiation aspects, yet there is a dearth of research examining these skills in relation to one another. The present study examined the relationship between these skillsets in women, exploring predictors of each. Initiation and refusal assertiveness were moderately correlated. Additionally, the relationship between them was not entirely explained by general assertiveness, indicating that there is something unique to assertiveness in the sexual context. Committed relationship context and erotophilic disposition specifically predicted initiation assertiveness. Less endorsement of the sexual double standard and fewer approach motivations for engaging in unwanted sex specifically predicted refusal assertiveness. Few differences emerged in predictors of assertiveness types when comparing sexual orientation groups, yet non-heterosexual women reported slightly lower levels of refusal assertiveness. Implications for sexual education, therapy, and future research are discussed. Author Keywords: sexual assertiveness, sexual autonomy, sexual compliance, sexual double standard, sexual satisfaction, sexual self-disclosure
mechanistic analysis of density dependence in algal population dynamics
Population density regulation is a fundamental principle in ecology, however there remain several unknowns regarding the functional expression of density dependence. One prominent view is that the patterns by which density dependence is expressed are largely fixed across a species, irrespective of environmental conditions. Our study investigated the expression of density dependence in Chlamydomonas reinhartti grown under a gradient of nutrient densities, and hypothesized that the relationship between per capita growth rate (pgr) and population density would vary from concave-up to concave-down as nutrients became less limiting. Contrary to prediction, we found that the relationship between a population's pgr and density became increasingly concave-up as nutrient levels increased. Our results suggest that density dependence is strongly variable depending on exogenous and endogenous processes acting on the population, implying that expression of density regulation depends extensively on local conditions. Population growth suppression may be attributable to environments with high intraspecific competition. Additional work should reveal the mechanisms influencing how the expression of density dependence varies across populations through space and time. Author Keywords: Chlamydomonas reinhartti, density dependence, logistic model, population dynamics, single species growth, theta-logistic equation
Assessment of an adult lake sturgeon translocation (Acipenser fulvescens) reintroduction effort in a fragmented river system
North American freshwater fishes are declining rapidly due to habitat fragmentation, degradation, and loss. In some cases, translocations can be used to reverse local extirpations by releasing species in suitable habitats that are no longer naturally accessible. Lake sturgeon (Acipenser fulvescens) experienced historical overharvest across their distribution, leading to endangered species listings and subsequent protection and recovery efforts. Despite harvest and habitat protections, many populations do not appear to be recovering, which has been attributed to habitat alteration and fragmentation by dams. In 2002, 51 adult lake sturgeon from the Mattagami River, Ontario, Canada were translocated 340 km upstream to a fragmented 35 km stretch of the river between two hydroelectric generating stations, where sturgeon were considered extirpated. This study assessed the translocation effort using telemetry (movement), demographics and genetic data. Within the first year, a portion of the radio-tagged translocated individuals dispersed out of the release area, and released radio-tagged individuals used different areas than individuals radio-tagged ten years later. Catches of juvenile lake sturgeon have increased over time, with 150 juveniles caught within the duration of this study. The reintroduced population had similar genetic diversity as the source population, with a marked reduction in effective population size (Ne). The results indicate that the reintroduction effort was successful, with evidence of successful spawning and the presence of juvenile lake sturgeon within the reintroduction site. Overall, the results suggest adult translocations may be a useful tool for re-establishing other extirpated lake sturgeon populations. Author Keywords: conservation, endangered species, lake sturgeon, reintroduction, telemetry, translocation
Observation-based assessment of atmospheric sulphur surrounding a major aluminum smelter in British Columbia, Canada
Recent developments at an aluminum (Al) smelter in Kitimat, BC resulted in a permitted increase of 27 to 42 tonnes of sulphur dioxide (SO2) emissions per day. Gaseous SO2 is a pollutant known to contribute to acidic deposition through processes of wet and dry deposition and can additionally react in-atmosphere to form particulate sulphate (pSO42-). Between June 2017 to October 2018, an extensive network consisting of ion exchange resin (IER) column, passive-diffusive, and active filter-pack samplers was established to provide an estimate of total annual S deposition and pSO42- variation throughout the Kitimat Valley. Filter-pack sampling determined the relative concentration of pSO42- increased downwind of the smelter. Comparison of observation-based and modelled total annual deposition suggested CALPUFF was accurate in representing the spatial viability of S deposition (R2 = > 0.85). However, the model appeared to overpredict near-field deposition suggesting the potential of underestimation further downwind of the smelter. Author Keywords: aluminum smelter, atmospheric deposition, filter-pack sampler, ion-exchange column sampler, pSO42-, SO2
Effects of Silver Nanoparticles on Lake Bacterioplankton
Silver nanoparticles (AgNP) released into aquatic environments could threaten natural bacterial communities and ecosystem services they provide. We examined natural lake bacterioplankton communities' responses to different exposures (pulse vs chronic) and types (citrate and PVP) of AgNPs at realistic environmental conditions using a mesocosm study at the Experimental Lakes Area. An in situ bioassay examined interactions between AgNPs and phosphorus loading. Bacterial communities exposed to high AgNP concentrations regardless of exposure or capping agent type accumulated silver. We observed increases in community production during additions of polyvinylpyrrolidone (PVP) -capped AgNPs and that site and nutrient-specific conditions are important to AgNPs toxicology in aquatic systems. Toxicological effects of AgNP are attenuated in natural conditions and differ from results from laboratory studies of AgNP toxicity. Our results demonstrate more studies are needed to fully assess the risk posed by these novel chemicals to the environment. This work could be useful in forming risk assessment policies which are largely based on lab studies and typically demonstrate strong toxic effects. Author Keywords: bacterial production, bacterioplankton communities, ecological stoichiometry, Experimental Lakes Area, mesocosms, silver nanoparticles
Seasonal habitat use and movement of native brook trout (Salvelinus fontinalis) in urban headwater streams
Coldwater streams are becoming increasingly impacted due to urbanization. Using environmental surveys, mark-recapture and telemetry, I assessed factors influencing seasonal brook trout (Salvelinus fontinalis) habitat use and movement in urban headwater streams in central Ontario between 2017-18. Generalized additive models were used to assess which habitat variables best explained seasonal yearling and older brook trout abundance, while generalized least squares models were used to assess overall trends in radio-tagged brook trout movement. My research demonstrated dynamic patterns in habitat use and movement by urban stream-dwelling brook trout. Yearlings were primarily influenced by water quality (stream temperature, conductivity), while older brook trout were most strongly influenced by stream morphology (depth, undercut bank). Movement occurred disproportionately around the spawning season and was more limited in the smaller, more altered stream. These findings may be used to inform fisheries managers on crucial timing and location of brook trout habitat refugia within urbanized environments. Author Keywords: Brook trout, coldwater stream, groundwater, habitat use, radiotelemetry, urbanization
Nitrogen Retention of Terricolous Lichens in a Jack Pine Forest in Northern Alberta
The Athabasca Oil Sands in Alberta, Canada is one of the largest point sources emitters of NOx in Canada and there are concerns that elevated nitrogen (N) deposition will lead to widespread eutrophication impacts, including altered species composition, similar to what has occurred in several parts of Europe. Atmospheric deposition rates as high as 25 kg N ha-1 yr-1 have been measured close to the industrial center. The role of the forest floor in regulating these potential eutrophication effects was investigated following a 5-year enrichment study in which N was applied as NH4NO3 above the canopy of a jack pine (Pinus banksiana Lamb) stand in northern Alberta close to Fort McMurray at dosages ranging from 5 – 25 kg N ha-1 yr-1 in addition to background deposition of 2 kg N ha-1 yr-1. Chemical analysis of lichen mats revealed that apical (upper) lichen tissue N concentration increased with treatment, as did the necrotic tissue. When expressed as a pool, the fibric-humic (FH) material held the largest quantity of N across all treatments due to its relatively large mass. Soil net N mineralization and net nitrification rates did not differ among N inputs after five years of application. A 15N tracer applied to the forest floor showed that N is initially absorbed by the apical lichen, FH material, and the foliage of the vascular plant Vaccinium myrtilloides in particular. After 2 years, the FH 15N pool size was elevated and all other measured pools were depleted, indicating a slow transfer of N to the FH material. Applied 15N was not detectable in mineral soil. The microbial functional gene ammonia monooxygenase (amoA) was undetectable using PCR screening of mineral soil microbial communities in all treatments, and broad fungal/bacterial qPCR assays revealed a weak treatment effect on fungal/bacterial ratios in mineral soil. This work suggests that terricolous lichen mats, which form the majority of ground cover in upland jack pine systems, have a large capacity to effectively retain elevated N deposition via the formation of stable humus. Author Keywords: Biogeochemistry, Boreal Ecology, Lichen, Nitrogen Enrichment, Oil Sands

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Format: 2023/02/09