Graduate Theses & Dissertations

Proximal Soil Nutrient Sensing in Croplands through Multispectral Imaging from Unmanned Aerial Vehicles (UAV) for Precision Agriculture Applications
Currently, UAVs are deployed to measure crop health in a timely manner by mapping vegetation indices. A study using two different fields was conducted in order to search for a relationship that may exist between crop health and soil fertility. A UAV equipped with sensor technology was used for mapping of vegetation indices which were then statistically compared to soil nutrient data collected via soil sampling. Elevation data was also collected which was then statistically compared to soil nutrients as well as crop health. Results of this study were unfortunately impacted by variables outside of the researcher’s control. Moisture became the greatest limiting factor in 2016 followed by an excess of rain in 2017. Results did not show any promising correlations as moisture uncontrollably became the defining variable. Further research in a more controlled setting will need to be conducted in order to explore this potential relationship. Author Keywords: Agriculture, Multispectral Imagery, Precision Agriculture, Proximal Soil Sensing, Remote Sensing, Unmanned Aerial Vehicle
Cytokinins in nematodes
To investigate cytokinins (CKs) in nematodes, CK profiles of a free-living Caenorhabditis elegans and a plant parasitic Heterodera glycines (soybean cyst nematode, SCN) were determined at the egg and larval stages. SCN had higher total CK level than C. elegans; however, CKs in SCN were mostly inactive precursors, whereas C. elegans had more bioactive forms. This is the first study to show that methylthiols are present in nematodes and may affect plant infection. In infectious SCN larvae, methylthiol levels were much higher than in eggs or C. elegans larvae. Furthermore, The CK profiles of SCN-susceptible and resistant Glycine max cultivars at three developmental stages revealed that, regardless of the resistance level, SCN infection caused an increase in root CKs. One resistant cultivar, Pion 93Y05, showed significantly high levels of bioactive N6-isopentenyladenine (iP) in the non-infected roots which indicated a potential role of CKs in soybean resistance to SCN. Author Keywords: Cytokinins, HPLC-MS/MS, Nematode, SCN resistance, Soybean

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