Graduate Theses & Dissertations

My Canadian Story
Canada prides itself on being a multicultural nation, but the stories of people who are not “Canadian-Canadians,” as defined by Eva Mackey, are underrepresented in archives. This project investigates three local archives and one online archive in Peterborough, Ontario, employing Rita Dhamoon’s practice of “accounts of meaning-making” to understand how archives contribute to a community’s understanding of itself and who belongs there. The findings indicate that the city’s “Canadian-Canadians,” who have portrayed them as transient and only temporarily settled in the city, frequently mediate the stories of “other” populations in Peterborough’s archival records. This account of meaning-making provides an entry point for changing this understanding and making archives more welcoming and accessible in the city and beyond. Author Keywords: Archives, Community, Identity, Immigration, Integration, Multiculturalism
CTRL + ALT + DEL
With the expansion of the internet, there are a number of opportunities to engage in cyber-bullying behaviour, however, to date, only a few studies have examined interpersonal predictors of cyber-bullying. The purpose of this research study was to explore relationship and personality factors associated with being a bully and/or a victim. The first goal of this study was to develop a comprehensive cyber-bullying measure. Results indicated three groups of cyber-bullying behaviours, including traditional (e.g. gossip); personal attack (e.g. negative remarks towards religion); and malicious behaviours (e.g. threats). Next, the associations between cyber-bullying and attachment, interdependence, and the dark triad of personality were examined. Analyses revealed that cyber-bullying was negatively associated with attachment security and interdependence and positively associated with insecurity and psychopathy. Discussion of the findings highlighted the importance of the dark triad in understanding predictors of cyber-bullying behaviours. Author Keywords: Attachment, Bullying, Cyber-bullying, Dark Triad, Interpersonal Relationships, Personality
Fungi and Cytokinins
Cytokinin biosynthesis in organisms aside from plant species has often been viewed as a byproduct of tRNA degradation. Recent evidence suggests that these tRNA degradation products may actually have a role in the development of these organisms, particularly fungi. This thesis examines the importance of cytokinins, a group of phytohormones involved in plant cell division and differentiation as well as the phytohormone abscisic acid, involved in plant response to environmental factors, and their presence and role in fungi. An initial survey was conducted on 20 temperate forest fungi of differing nutritional modes. Using HPLC-ESI MS/MS, cytokinin and abscisic acid were detected in all fungi regardless of their mode of nutrition or phylogeny. The detection of the same seven CKs across all fungi suggested the existence of a common CK biosynthetic pathway and dominance of the tRNA pathway in fungi. Further, the corn smut fungus Ustilago maydis is capable of producing CKs separate from its host and different U. maydis strains induce disease symptoms of differing severity. To determine if CK production during infection alters disease development a disease time course was conducted on cob tissue infected with U. maydis dikaryotic and solopathogenic strains. Dramatic changes in phytohormones including an increase in ABA followed by increases in cisZCKs were detected in tumour tissue particularity in the more virulent dikaryon infection, suggesting a role for CKs in strain virulence. Mining of the U. maydis genome identified a sole tRNA-isopentenyltransferase, a key enzyme in CK biosynthesis. Targeted gene deletion mutants were created in U. maydis which halted U. maydis CK production and decreased pathogenesis and virulence in seedling and cob infections. CK and ABA profiling carried out during disease development found that key changes in these hormones were not found in deletion mutant infections and cob tumour development was severely impaired. These findings suggested that U. maydis CK production is necessary for tumour development in this pathosystem. The research presented in this thesis highlights the importance of fungal CKs, outlines the dominant CK pathway in fungi, identifies a key enzyme in U. maydis CK biosynthesis and reveals the necessity of CK production by U. maydis in the development of cob tumours. Author Keywords: abscisic acid, cytokinins, high performance liquid chromatography-electrospray ionization tandem mass spectrometry, tRNA degradation pathway, Ustilago maydis, Zea mays
Learning From One Another
Biomaterial technology and utilizing bioproducts can contribute to Canada's economic growth while moving towards sustainable development. Canadian bioproducts are commonly developed within universities but Canada's record of transferring university technology to market has been less than optimal. In an attempt to offer new ideas for improvement, qualitative data analysis from comparing stakeholder interviews in Canada and Brazil regarding university technology transfer through biomaterial spin-off development identifies the enablers and barriers to success. This thesis offers modality changes that if implemented will contribute to increasing university spin-off development in Canada to achieve economic growth and sustainable development. These modality changes include: 1) Create research network alliances; 2) Incorporate university commercialization activities into faculty performance measurement; 3) Implement a general business class as a pre-requisite to all degree requirements; 4) Restructure funding programs from one time sums to phase based implementation; 5) Establish a pre-incubation program in addition to the traditional incubator. Author Keywords: biomaterials, Brazil, Canada, policy, university spin-off, university technology transfer

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